ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAKEAVKYVW EGAIPLQIHL HKSDVASHPA PPPALVLAPR IGYLPLLIPL IKPYFKDSLP 

        70         80         90        100        110        120 
PGEDSIWFDY KGFPLKWYIP TGVLFDLLCA EPERPWNLTI HFRGYPCNIL IPCEGEDSVK 

       130        140        150        160        170        180 
WNFVNSLKEA QYIINGNCKN VMNMSQSDQE DLWTSVMNGD LDAYTRLSPK LKMGTVEDEF 

       190        200        210        220        230        240 
SRKTSLSSPQ SQQVVPETEV AGQVKTARIP VRLYVRSLNK DFENLEDVPE IDTWDDISYL 

       250        260        270        280        290        300 
NRPVEFLKEE GKCFTLRDAI KSLLPEFMGD RAQTSGEERS IDDTEEADGS REMGEIKLVR 

       310        320        330 
IQGIEMKLEI PFSWVVNNLM NPEFYLHISV LVKAPQR 

References and documentation are available.

Number of amino acids: 337

Molecular weight: 38477.09

Theoretical pI: 4.95

Amino acid composition: 
Ala (A)  16	  4.7%
Arg (R)  15	  4.5%
Asn (N)  15	  4.5%
Asp (D)  21	  6.2%
Cys (C)   5	  1.5%
Gln (Q)  11	  3.3%
Glu (E)  29	  8.6%
Gly (G)  17	  5.0%
His (H)   5	  1.5%
Ile (I)  22	  6.5%
Leu (L)  34	 10.1%
Lys (K)  21	  6.2%
Met (M)   9	  2.7%
Phe (F)  13	  3.9%
Pro (P)  27	  8.0%
Ser (S)  23	  6.8%
Thr (T)  12	  3.6%
Trp (W)   8	  2.4%
Tyr (Y)  11	  3.3%
Val (V)  23	  6.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 50
Total number of positively charged residues (Arg + Lys): 36

Atomic composition:

Carbon      C	      1739
Hydrogen    H	      2699
Nitrogen    N	       447
Oxygen      O	       510
Sulfur      S	        14

Formula: C1739H2699N447O510S14
Total number of atoms: 5409

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    60640
Abs 0.1% (=1 g/l)   1.576, assuming all pairs of Cys residues form cystines


Ext. coefficient    60390
Abs 0.1% (=1 g/l)   1.570, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.80
This classifies the protein as unstable.



Aliphatic index: 89.35

Grand average of hydropathicity (GRAVY): -0.327