ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAKEDTGVTA PKKPETAQVA NINGIDKLEP PKTKEETESS KSVSSEKAAH ASDESFKRSI 

        70         80         90        100        110        120 
HEASYVGWKQ IGGWEDKDEL TLDDELMDMT RETFLDNIIP DSLYGDWYHS VAIFFIGGVA 

       130        140        150        160        170        180 
SFALGHYKFS MGSAFFVIVI TSLLYRTSAK KYRGSIRELV QKEFTVQKVE NDYESLEWLN 

       190        200        210        220        230        240 
AFLDKYWPIL EPSVSQLIVQ QANEQMATNE AIPKFITQLW IDELTLGVKP PRVDLVKTFQ 

       250        260        270        280        290        300 
NTASDVVVMD WGISFTPHDL CDMSAKQVRN YVNELAVVKA KIFGITIPVS VSDIAFKAHA 

       310        320        330        340        350        360 
RVKFKLMTPF PHVETVNIQL LKVPDFDFVA TLFGRSIFNW EILAIPGLMT LIQKMAKKYM 

       370        380        390        400        410        420 
GPILLPPFSL QLNIPQLLSG SNLSIGILEI TVKNAKGLKR TSSILNESID PYLSFEFNDI 

       430        440        450        460        470        480 
SIAKTRTVRD TLNPVWDETL YVLLNSFTDP LTISVYDKRA KLKDKVLGRI QYNLNTLHDK 

       490        500        510        520        530        540 
TTQRNLKAQF LRNSKPVGEL TFDLRFFPTL EEKKLPDGSV EELPDLNTGI AKVVVEEGSR 

       550        560        570        580        590        600 
FAEEEQKVTA YVEVYLNAKL VLTTGKATDT GTLKWNSDYE AVIADRRKTR YKFVVKDGKG 

       610        620        630        640        650        660 
EEIGSTIQTL NDLIDRSQVN KNLIPLKNQK GDIKITTYWR PVRLEIGSNS VAYTPPIGAI 

       670        680        690        700        710        720 
RVFIEKANDL RNLEKFGTID PYCKVLVNGL SKGRTDFKSQ TLNPVWNQVI YVAVTSPNQR 

       730        740        750        760        770        780 
ITLQCMDVET VNKDRSLGEF NVNVQDLFKK DENDKYEETI DEKAKVGRLV MPKKKPKGTI 

       790        800        810        820        830        840 
TYYTSFYPAL PVLTLEEIQD LDKVNKKKKA LELRKSAIDE KKISKEDKAK FDQEWNEVKE 

       850        860        870        880        890        900 
LEDMYSNRQK LDLPELLQYN QGVLAVTVLN GELPDSGLYV QAFFDDNGHP RFVSPRIPSR 

       910        920        930        940        950        960 
IVKNGWSGDV IIKELDKSIT TFRVAKNKNY NRVEKCVCEV ELPTQELVKN CYYKPSILHL 

       970        980        990       1000       1010       1020 
SGEGSAKLML QISWFPIDTK QLPANDLITN SGDLTIMSRS AENLIASDLN GYSDPYLKYY 

      1030       1040       1050       1060       1070       1080 
INNEEDCAYK TKVVKKTLNP KWNDEGTIQI NNRLNDVLRI KVMDWDSTSA DDTIGTAEIP 

      1090       1100       1110       1120       1130       1140 
LNKVKVEGTT ELDVPVEGLE NAGQDGGMLH LAFSFKPRYT ISVSKREKKV GDIASKGLGT 

      1150       1160       1170       1180 
GLKAGTTVIG GGVGAIGKIK KGVFGGLGSL TNHKKNHEMG EEETKF 

References and documentation are available.

Number of amino acids: 1186

Molecular weight: 133576.03

Theoretical pI: 6.74

Amino acid composition: 
Ala (A)  62	  5.2%
Arg (R)  42	  3.5%
Asn (N)  67	  5.6%
Asp (D)  76	  6.4%
Cys (C)   7	  0.6%
Gln (Q)  38	  3.2%
Glu (E)  83	  7.0%
Gly (G)  71	  6.0%
His (H)  13	  1.1%
Ile (I)  79	  6.7%
Leu (L) 114	  9.6%
Lys (K) 115	  9.7%
Met (M)  19	  1.6%
Phe (F)  47	  4.0%
Pro (P)  52	  4.4%
Ser (S)  74	  6.2%
Thr (T)  80	  6.7%
Trp (W)  17	  1.4%
Tyr (Y)  38	  3.2%
Val (V)  92	  7.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 159
Total number of positively charged residues (Arg + Lys): 157

Atomic composition:

Carbon      C	      6013
Hydrogen    H	      9553
Nitrogen    N	      1575
Oxygen      O	      1802
Sulfur      S	        26

Formula: C6013H9553N1575O1802S26
Total number of atoms: 18969

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   150495
Abs 0.1% (=1 g/l)   1.127, assuming all pairs of Cys residues form cystines


Ext. coefficient   150120
Abs 0.1% (=1 g/l)   1.124, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 28.94
This classifies the protein as stable.



Aliphatic index: 91.19

Grand average of hydropathicity (GRAVY): -0.356