ProtParam
User-provided sequence:
10 20 30 40 50 60
MAKEPVRVLV TGAAGQIGYA LVPMIARGIM LGADQPVILH MLDIPPAAEA LNGVKMELID
70 80 90 100 110 120
AAFPLLKGVV ATTDAVEGCT GVNVAVMVGG FPRKEGMERK DVMSKNVSIY KSQAAALEKH
130 140 150 160 170 180
AAPNCKVLVV ANPANTNALI LKEFAPSIPE KNISCLTRLD HNRALGQISE RLSVPVSDVK
190 200 210 220 230 240
NVIIWGNHSS SQYPDVNHAK VQTSSGEKPV RELVKDDAWL DGEFISTVQQ RGAAIIKARK
250 260 270 280 290 300
LSSALSAASS ACDHIRDWVL GTPEGTFVSM GVYSDGSYSV PSGLIYSFPV TCRNGDWSIV
310 320 330
QGLPIDEVSR KKMDLTAEEL KEEKDLAYSC LS
References and
documentation are available.
Number of amino acids: 332
Molecular weight: 35570.97
Theoretical pI: 6.11
Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 34
Atomic composition:
Carbon C 1569
Hydrogen H 2538
Nitrogen N 430
Oxygen O 477
Sulfur S 16
Formula: C
1569H
2538N
430O
477S
16
Total number of atoms: 5030
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 32805
Abs 0.1% (=1 g/l) 0.922, assuming all pairs of Cys residues form cystines
Ext. coefficient 32430
Abs 0.1% (=1 g/l) 0.912, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 36.73
This classifies the protein as stable.
Aliphatic index: 96.63
Grand average of hydropathicity (GRAVY): 0.013