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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAKEPVRVLV TGAAGQIGYA LVPMIARGIM LGADQPVILH MLDIPPAAEA LNGVKMELID

70 80 90 100 110 120
AAFPLLKGVV ATTDAVEGCT GVNVAVMVGG FPRKEGMERK DVMSKNVSIY KSQAAALEKH

130 140 150 160 170 180
AAPNCKVLVV ANPANTNALI LKEFAPSIPE KNISCLTRLD HNRALGQISE RLSVPVSDVK

190 200 210 220 230 240
NVIIWGNHSS SQYPDVNHAK VQTSSGEKPV RELVKDDAWL DGEFISTVQQ RGAAIIKARK

250 260 270 280 290 300
LSSALSAASS ACDHIRDWVL GTPEGTFVSM GVYSDGSYSV PSGLIYSFPV TCRNGDWSIV

310 320 330
QGLPIDEVSR KKMDLTAEEL KEEKDLAYSC LS


References and documentation are available.

Number of amino acids: 332 Molecular weight: 35570.97 Theoretical pI: 6.11
Amino acid composition: 
Ala (A) 35 10.5% Arg (R) 13 3.9% Asn (N) 13 3.9% Asp (D) 18 5.4% Cys (C) 6 1.8% Gln (Q) 9 2.7% Glu (E) 19 5.7% Gly (G) 24 7.2% His (H) 6 1.8% Ile (I) 20 6.0% Leu (L) 28 8.4% Lys (K) 21 6.3% Met (M) 10 3.0% Phe (F) 6 1.8% Pro (P) 18 5.4% Ser (S) 29 8.7% Thr (T) 12 3.6% Trp (W) 4 1.2% Tyr (Y) 7 2.1% Val (V) 34 10.2% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 37 Total number of positively charged residues (Arg + Lys): 34 Atomic composition: Carbon C 1569 Hydrogen H 2538 Nitrogen N 430 Oxygen O 477 Sulfur S 16 Formula: C1569H2538N430O477S16 Total number of atoms: 5030 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 32805 Abs 0.1% (=1 g/l) 0.922, assuming all pairs of Cys residues form cystines Ext. coefficient 32430 Abs 0.1% (=1 g/l) 0.912, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 36.73 This classifies the protein as stable. Aliphatic index: 96.63 Grand average of hydropathicity (GRAVY): 0.013