ProtParam
User-provided sequence:
10 20 30 40 50 60
MAKETVCVTG ANGFIGSWII RTLIEKGYTK IHASIYPGSD PTHLLQLPGS DSKIKIFEAD
70 80 90 100 110 120
LLDSDAISRA IDGCAGVFHV ASPCTLDPPV DPEKELVEPA VKGTINVLEA AKRFNVRRVV
130 140 150 160 170 180
ITSSISALVP NPNWPEKVPV DESSWSDLDF CKSRQKWYPI SKTLAEKAAW EFSEKHGTNI
190 200 210 220 230 240
VTIHPSTCLG PLLQPNLNAS CAVLLQLLQG STETQEHHWL GVVHVKDVAK GHVMLFETPD
250 260 270 280 290 300
ASGRFLCTNG IYQFSEFAAL VSKLFPEFAV HKFDKETQPG LTSCNDAAKR LIELGLVFTA
310
VEDAVKETVQ SLRDKGFL
References and
documentation are available.
Number of amino acids: 318
Molecular weight: 34839.92
Theoretical pI: 5.82
Total number of negatively charged residues (Asp + Glu): 38
Total number of positively charged residues (Arg + Lys): 31
Atomic composition:
Carbon C 1570
Hydrogen H 2464
Nitrogen N 412
Oxygen O 463
Sulfur S 10
Formula: C
1570H
2464N
412O
463S
10
Total number of atoms: 4919
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 39460
Abs 0.1% (=1 g/l) 1.133, assuming all pairs of Cys residues form cystines
Ext. coefficient 38960
Abs 0.1% (=1 g/l) 1.118, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.31
This classifies the protein as stable.
Aliphatic index: 92.89
Grand average of hydropathicity (GRAVY): -0.049