ProtParam
User-provided sequence:
10 20 30 40 50 60
MAKGFKLKEL LSHQKEIEKA EKLENDLKKK KSQELKKEEP TIVTASNLKK LEKKEKKADV
70 80 90 100 110 120
KKEVAADTEE YQSQALSKKE KRKLKKELKK MQEQDATEAQ KHMSGDEDES GDDREEEEEE
130 140 150 160 170 180
EEEEEGRLDL EKLAKSDSES EDDSESENDS EEDEDVVAKE ESEEKEEQEE EQDVPLSDVE
190 200 210 220 230 240
FDSDADVVPH HKLTVNNTKA MKHALERVQL PWKKHSFQEH QSVTSETNTD EHIKDIYDDT
250 260 270 280 290 300
ERELAFYKQS LDAVLVARDE LKRLKVPFKR PLDYFAEMVK SDEHMDKIKG KLIEEASDKK
310 320 330 340 350 360
AREEARRQRQ LKKFGKQVQN ATLQKRQLEK RETLEKIKSL KNKRKHNEID HSEFNVGVEE
370 380 390 400 410 420
EVEGKRFDRG RPNGKRAAKN AKYGQGGMKR FKRKNDATSS ADVSGFSSRK MKGKTNRPGK
SRRARRF
References and
documentation are available.
Number of amino acids: 427
Molecular weight: 49734.36
Theoretical pI: 6.05
Total number of negatively charged residues (Asp + Glu): 104
Total number of positively charged residues (Arg + Lys): 97
Atomic composition:
Carbon C 2129
Hydrogen H 3481
Nitrogen N 635
Oxygen O 719
Sulfur S 8
Formula: C
2129H
3481N
635O
719S
8
Total number of atoms: 6972
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 12950
Abs 0.1% (=1 g/l) 0.260
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 63.23
This classifies the protein as unstable.
Aliphatic index: 55.53
Grand average of hydropathicity (GRAVY): -1.489