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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAKIMIPASK QPVYKKLGLL LVAVFTVYVF FHGAQYARGS APSPKYSTVL SSGSGYKYSK

70 80 90 100 110 120
VELPKYTGPR EKATFVTLVR NRDLYSLAES IKSVEDRFNS KFNYDWVFLN DEEFTDEFKN

130 140 150 160 170 180
VTSALVSGTT KYGVIPKEHW SFPEWIDEEK AAQVRKEMGE KRIIYGDSIS YRHMCRFESG

190 200 210 220 230 240
FFYRHPLMDD YDWYWRVEPD IKLHCDIDYD VFKFMKDNKK KYAFAISIKE YEATIPTLWE

250 260 270 280 290 300
TTRKFMEAHP ELIHENNMLD FVSDDQGLSY NLCHFWSNFE IAALDLWRSP AYSAYFDYLD

310 320 330 340 350 360
REGGFFYERW GDAPVHSIGA ALFLDRSEIH HFGDIGYYHV PFHSCPIDTS IRLANKCDCD

370 380 390
PSKDFTWHSY SCTTKFYNIN KLPKPAGWQN HIG


References and documentation are available.

Number of amino acids: 393 Molecular weight: 46022.07 Theoretical pI: 6.21
Amino acid composition: 
Ala (A) 24 6.1% Arg (R) 17 4.3% Asn (N) 14 3.6% Asp (D) 28 7.1% Cys (C) 7 1.8% Gln (Q) 5 1.3% Glu (E) 25 6.4% Gly (G) 20 5.1% His (H) 15 3.8% Ile (I) 22 5.6% Leu (L) 25 6.4% Lys (K) 30 7.6% Met (M) 8 2.0% Phe (F) 28 7.1% Pro (P) 19 4.8% Ser (S) 31 7.9% Thr (T) 17 4.3% Trp (W) 11 2.8% Tyr (Y) 27 6.9% Val (V) 20 5.1% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 53 Total number of positively charged residues (Arg + Lys): 47 Atomic composition: Carbon C 2117 Hydrogen H 3106 Nitrogen N 534 Oxygen O 594 Sulfur S 15 Formula: C2117H3106N534O594S15 Total number of atoms: 6366 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 101105 Abs 0.1% (=1 g/l) 2.197, assuming all pairs of Cys residues form cystines Ext. coefficient 100730 Abs 0.1% (=1 g/l) 2.189, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 39.66 This classifies the protein as stable. Aliphatic index: 67.51 Grand average of hydropathicity (GRAVY): -0.461