ProtParam
User-provided sequence:
10 20 30 40 50 60
MAKIMIPASK QPVYKKLGLL LVAVFTVYVF FHGAQYARGS APSPKYSTVL SSGSGYKYSK
70 80 90 100 110 120
VELPKYTGPR EKATFVTLVR NRDLYSLAES IKSVEDRFNS KFNYDWVFLN DEEFTDEFKN
130 140 150 160 170 180
VTSALVSGTT KYGVIPKEHW SFPEWIDEEK AAQVRKEMGE KRIIYGDSIS YRHMCRFESG
190 200 210 220 230 240
FFYRHPLMDD YDWYWRVEPD IKLHCDIDYD VFKFMKDNKK KYAFAISIKE YEATIPTLWE
250 260 270 280 290 300
TTRKFMEAHP ELIHENNMLD FVSDDQGLSY NLCHFWSNFE IAALDLWRSP AYSAYFDYLD
310 320 330 340 350 360
REGGFFYERW GDAPVHSIGA ALFLDRSEIH HFGDIGYYHV PFHSCPIDTS IRLANKCDCD
370 380 390
PSKDFTWHSY SCTTKFYNIN KLPKPAGWQN HIG
References and
documentation are available.
Number of amino acids: 393
Molecular weight: 46022.07
Theoretical pI: 6.21
Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 47
Atomic composition:
Carbon C 2117
Hydrogen H 3106
Nitrogen N 534
Oxygen O 594
Sulfur S 15
Formula: C
2117H
3106N
534O
594S
15
Total number of atoms: 6366
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 101105
Abs 0.1% (=1 g/l) 2.197, assuming all pairs of Cys residues form cystines
Ext. coefficient 100730
Abs 0.1% (=1 g/l) 2.189, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 39.66
This classifies the protein as stable.
Aliphatic index: 67.51
Grand average of hydropathicity (GRAVY): -0.461