ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAKKVAICTL LYSRDYLPGA LTLAYQLQKL LKHAVVEDEI TLCLLIEKKL FGDEFKPQEI 

        70         80         90        100        110        120 
ALIRSLFKEI IIIEPLKDQE KSIEKNKANL ELLKRPELSH TLLKARLWEL VQFDQVLFLD 

       130        140        150        160        170        180 
ADTLPLNKEF FEILRLYPEQ TRFQIAAVPD IGWPDMFNTG VLLLIPDLDM ATSLQDFLIK 

       190        200        210        220        230        240 
TVSIDGADQG IFNQFFNPIC NYSKEVLHKV SPLMEWIRLP FTYNVTMPNY GYQSSPAMNF 

       250        260        270        280        290        300 
FQQHIRLIHF IGTFKPWSRN TTDYDDHYYQ LWRSTQRELY SECHLSNYFT HLQLGNIETE 

       310        320        330        340        350        360 
TNFYHEPPCL QDLLNHGKRE NQKHVDLDIT SVDRNASQKS TAEKHDIEKP TSKPQSAFKF 

       370        380 
DWESTDYLDR VQRAFPKPDT 

References and documentation are available.

Number of amino acids: 380

Molecular weight: 44546.02

Theoretical pI: 5.68

Amino acid composition: 
Ala (A)  18	  4.7%
Arg (R)  15	  3.9%
Asn (N)  17	  4.5%
Asp (D)  26	  6.8%
Cys (C)   5	  1.3%
Gln (Q)  22	  5.8%
Glu (E)  26	  6.8%
Gly (G)  10	  2.6%
His (H)  12	  3.2%
Ile (I)  25	  6.6%
Leu (L)  49	 12.9%
Lys (K)  27	  7.1%
Met (M)   6	  1.6%
Phe (F)  23	  6.1%
Pro (P)  21	  5.5%
Ser (S)  20	  5.3%
Thr (T)  23	  6.1%
Trp (W)   6	  1.6%
Tyr (Y)  15	  3.9%
Val (V)  14	  3.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 52
Total number of positively charged residues (Arg + Lys): 42

Atomic composition:

Carbon      C	      2034
Hydrogen    H	      3129
Nitrogen    N	       521
Oxygen      O	       582
Sulfur      S	        11

Formula: C2034H3129N521O582S11
Total number of atoms: 6277

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    55600
Abs 0.1% (=1 g/l)   1.248, assuming all pairs of Cys residues form cystines


Ext. coefficient    55350
Abs 0.1% (=1 g/l)   1.243, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.86
This classifies the protein as unstable.



Aliphatic index: 91.37

Grand average of hydropathicity (GRAVY): -0.384