ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAKLVHDVQK KQHRERSQLT SRSRYGFLEK HKDYVKRAQD FHRKQSTLKV LREKAKERNP 

        70         80         90        100        110        120 
DEYYHAMHSR KTDAKGLLIS SRHGDEEDES LSMDQVKLLK TQDSNYVRTL RQIELKKLEK 

       130        140        150        160        170        180 
GAKQLMFKSS GNHTIFVDSR EKMNEFTPEK FFNTTSEMVN RSENRLTKDQ LAQDISNNRN 

       190        200        210        220        230        240 
ASSIMPKESL DKKKLKKFKQ VKQHLQRETQ LKQVQQRMDA QRELLKKGSK KKIVDSSGKI 

       250 
SFKWKKQRKR 

References and documentation are available.

Number of amino acids: 250

Molecular weight: 29735.10

Theoretical pI: 10.18

Amino acid composition: 
Ala (A)   9	  3.6%
Arg (R)  21	  8.4%
Asn (N)  10	  4.0%
Asp (D)  15	  6.0%
Cys (C)   0	  0.0%
Gln (Q)  20	  8.0%
Glu (E)  18	  7.2%
Gly (G)   7	  2.8%
His (H)   9	  3.6%
Ile (I)   7	  2.8%
Leu (L)  22	  8.8%
Lys (K)  41	 16.4%
Met (M)   8	  3.2%
Phe (F)   9	  3.6%
Pro (P)   3	  1.2%
Ser (S)  23	  9.2%
Thr (T)  11	  4.4%
Trp (W)   1	  0.4%
Tyr (Y)   5	  2.0%
Val (V)  11	  4.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 33
Total number of positively charged residues (Arg + Lys): 62

Atomic composition:

Carbon      C	      1291
Hydrogen    H	      2135
Nitrogen    N	       403
Oxygen      O	       386
Sulfur      S	         8

Formula: C1291H2135N403O386S8
Total number of atoms: 4223

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    12950
Abs 0.1% (=1 g/l)   0.436

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.68
This classifies the protein as unstable.



Aliphatic index: 61.60

Grand average of hydropathicity (GRAVY): -1.308