ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAKMGLKPDP ATTNQTHNNA KEIRYRGVRK RPWGRYAAEI RDPGKKTRVW LGTFDTAEEA 

        70         80         90        100        110        120 
ARAYDTAARD FRGAKAKTNF PTFLELSDQK VPTGFARSPS QSSTLDCASP PTLVVPSATA 

       130        140        150        160        170        180 
GNVPPQLELS LGGGGGGSCY QIPMSRPVYF LDLMGIGNVG RGQPPPVTSA FRSPVVHVAT 

       190        200        210        220 
KMACGAQSDS DSSSVVDFEG GMEKRSQLLD LDLNLPPPSE QA 

References and documentation are available.

Number of amino acids: 222

Molecular weight: 23740.79

Theoretical pI: 9.01

Amino acid composition: 
Ala (A)  22	  9.9%
Arg (R)  15	  6.8%
Asn (N)   7	  3.2%
Asp (D)  13	  5.9%
Cys (C)   3	  1.4%
Gln (Q)   9	  4.1%
Glu (E)   9	  4.1%
Gly (G)  21	  9.5%
His (H)   2	  0.9%
Ile (I)   4	  1.8%
Leu (L)  16	  7.2%
Lys (K)  11	  5.0%
Met (M)   6	  2.7%
Phe (F)   8	  3.6%
Pro (P)  21	  9.5%
Ser (S)  19	  8.6%
Thr (T)  15	  6.8%
Trp (W)   2	  0.9%
Tyr (Y)   5	  2.3%
Val (V)  14	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 22
Total number of positively charged residues (Arg + Lys): 26

Atomic composition:

Carbon      C	      1036
Hydrogen    H	      1642
Nitrogen    N	       300
Oxygen      O	       322
Sulfur      S	         9

Formula: C1036H1642N300O322S9
Total number of atoms: 3309

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    18575
Abs 0.1% (=1 g/l)   0.782, assuming all pairs of Cys residues form cystines


Ext. coefficient    18450
Abs 0.1% (=1 g/l)   0.777, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 63.58
This classifies the protein as unstable.



Aliphatic index: 63.33

Grand average of hydropathicity (GRAVY): -0.483