ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAKRLLESSQ NDQANRKNSK IEKKEVSFYE EEETDDSFDS FYQDEEDDLS DIDWEEVSLD 

        70         80         90        100        110        120 
GSLTVTFGNI RRDREKVSKY KRKHNKKAFN YQRLKYGLHL IMIPFMLFLL KSRMKWIDDE 

       130        140        150        160        170        180 
RLNRRLRRSV PKLIGKKFKD WDVRDPAFKM DSLRTLLLGL VLWFRSNYKM NSNGIRQNFN 

       190        200        210        220        230        240 
RLQYLIKYAD NQNENSISES TYKKVLENQQ EFYGNRPLIN HGVEDIRKMA KRKMANRDIL 

       250        260        270        280        290        300 
TLFFFIILEN VLPGPKKLYL CFALPLHDYD IRCNKVKWQI EHGIGKVPNR FDSDLIQPYF 

       310        320        330        340        350        360 
WIELEVPTLS DGELYIIDPI AHLGEREMVL KTREDQFVPT YQPSVDMKYN LNQKFHYVVR 

       370        380        390        400        410        420 
INHAEKVLQD VSPRYVPNVC YRYFELSESS PILKSKHYTS YQYLSKWLKV LNKKKASVHH 

       430        440        450        460        470        480 
YAIMKKIALT NFTLPKSVTE IKRTDNFVIP SLLKSNEVLK ACAKQAATFT KGDNSQEPIF 

       490        500        510        520        530        540 
WRRDVIQLKS KQHWAILGRS ILPNAQPLKR KKYLPMRERM VRNLDKYVIK ELFSYEQTMK 

       550        560        570        580        590        600 
SPKYPSTYCD HLGQEHVITD LSHYKNKFGN IEIYSKETKP DGFELIPLSK EVDIKCLIKE 

       610        620        630        640        650        660 
YNKGKRKMQK IKYLDVVSGF DFKQKKGHAI PKIESILVKE TDYKAVQLLK QQTKVLLGLS 

       670        680        690 
FWDILLRKLR VNDRLNAEYG NVGNNEETLD DH 

References and documentation are available.

Number of amino acids: 692

Molecular weight: 82042.77

Theoretical pI: 9.45

Amino acid composition: 
Ala (A)  22	  3.2%
Arg (R)  41	  5.9%
Asn (N)  41	  5.9%
Asp (D)  44	  6.4%
Cys (C)   6	  0.9%
Gln (Q)  28	  4.0%
Glu (E)  46	  6.6%
Gly (G)  24	  3.5%
His (H)  17	  2.5%
Ile (I)  45	  6.5%
Leu (L)  74	 10.7%
Lys (K)  80	 11.6%
Met (M)  15	  2.2%
Phe (F)  31	  4.5%
Pro (P)  27	  3.9%
Ser (S)  44	  6.4%
Thr (T)  23	  3.3%
Trp (W)  10	  1.4%
Tyr (Y)  35	  5.1%
Val (V)  39	  5.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 90
Total number of positively charged residues (Arg + Lys): 121

Atomic composition:

Carbon      C	      3717
Hydrogen    H	      5856
Nitrogen    N	      1008
Oxygen      O	      1044
Sulfur      S	        21

Formula: C3717H5856N1008O1044S21
Total number of atoms: 11646

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   107525
Abs 0.1% (=1 g/l)   1.311, assuming all pairs of Cys residues form cystines


Ext. coefficient   107150
Abs 0.1% (=1 g/l)   1.306, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.14
This classifies the protein as unstable.



Aliphatic index: 86.59

Grand average of hydropathicity (GRAVY): -0.648