ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAKRNRSQFR IQERTAKKRK HEDSLLEGNV FQNAPEDMDE NTIYSAKGSS WDEEEQDYEM 

        70         80         90        100        110        120 
VPRKNRSDTS NLVEGLPIKV NGKVERKLHK AQEKPKDDDE EDEDSNDSSE DDEGPNEEQE 

       130        140        150        160        170        180 
AEAKEDEPDT EEKILQLKED IADLVTKVME EPEENTAALG RLCKMVESKN PNTCKFSMLA 

       190        200        210        220        230        240 
LVPVFKSIIP GYRIRPLTET EKKEKVSKEV SKLRNFEQAL VYNYKNYVGR LQSLSKTPSN 

       250        260        270        280        290        300 
AAPIQVSLGI LATQAAKELI STASHFNFRT DIFTLLLRRI CKPRISTDPT SIQIIQTFET 

       310        320        330        340        350        360 
LLNEDEEGSI SFEILRIFNK ILKTRNFNIE ESVLNMLLSL DVLHDYDPNT KLKGNVSAPK 

       370        380        390        400        410        420 
LKKKDRVHLS KKQRKARKEM QQIEEEMRNA EQAVSAEERE RNQSEILKIV FTIYLNILKN 

       430        440        450        460        470        480 
NAKTLIGSVL EGLTKFGNMA NFDLLGDFLE VMKELISDTE FDNLSSAEVR KALLCIVSAF 

       490        500        510        520        530        540 
SLISNTQYMK VNVDLSKFVD GLYALLPYIC LDADIELSYR SLRLADPLNN EIIKPSVNVS 

       550        560        570        580        590        600 
TKAELLLKAL DHVFFRSKSG TKERATAFTK RLYMCISHTP EKTSIAILKF IDKLMNRYPE 

       610        620        630        640        650        660 
ISGLYSSEDR IGNGHFIMEA DNPSRSNPEA ATLWDNALLE KHYCPVVTKG LRSLSSRSKE 


CSK 

References and documentation are available.

Number of amino acids: 663

Molecular weight: 75582.05

Theoretical pI: 5.73

Amino acid composition: 
Ala (A)  40	  6.0%
Arg (R)  36	  5.4%
Asn (N)  42	  6.3%
Asp (D)  39	  5.9%
Cys (C)   8	  1.2%
Gln (Q)  19	  2.9%
Glu (E)  69	 10.4%
Gly (G)  21	  3.2%
His (H)   9	  1.4%
Ile (I)  42	  6.3%
Leu (L)  71	 10.7%
Lys (K)  62	  9.4%
Met (M)  15	  2.3%
Phe (F)  24	  3.6%
Pro (P)  25	  3.8%
Ser (S)  57	  8.6%
Thr (T)  33	  5.0%
Trp (W)   2	  0.3%
Tyr (Y)  16	  2.4%
Val (V)  33	  5.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 108
Total number of positively charged residues (Arg + Lys): 98

Atomic composition:

Carbon      C	      3320
Hydrogen    H	      5372
Nitrogen    N	       914
Oxygen      O	      1047
Sulfur      S	        23

Formula: C3320H5372N914O1047S23
Total number of atoms: 10676

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    35340
Abs 0.1% (=1 g/l)   0.468, assuming all pairs of Cys residues form cystines


Ext. coefficient    34840
Abs 0.1% (=1 g/l)   0.461, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.38
This classifies the protein as unstable.



Aliphatic index: 86.94

Grand average of hydropathicity (GRAVY): -0.571