ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAKRPLGLGK QSREKKRKVE SVEKKSDEPS RESTPVRSQM SVELDDDADL DDELAQLKGL 

        70         80         90        100        110        120 
WSKYFHSDRD DEYVLNGIVH ECDRLLRLSE EDKEIKKTLN DIFHGIYALA LSELTIFKAG 

       130        140        150        160        170        180 
DEEATEEKRK KDVSSFFESA IERVELGLSH FPESQFLKLV LAKIIFQRIP LEYISNLHLK 

       190        200        210        220        230        240 
SKDKKLDLVG QLEHGKKHFS IYENDTEFTF EILQMVNDLL DIVENFGREQ SIQEGIDSDN 

       250        260        270        280        290        300 
EEEEELIDIE LEPEHPVYPL QQSLEANYEW LRNHFDKLLD NTNTDVKIYA SIANTLGELY 

       310        320        330        340        350        360 
LKKAEEPSKV FLSLQYDDGG SEKVSDKEAK NAQETALKHT KKALEYLEKA KLEDDPDTWV 

       370        380        390        400        410 
QVAEAYIDLG NLLDNESAEQ EEAYKTAEEI LGKANKASHG KFQDVLDNFL QG 

References and documentation are available.

Number of amino acids: 412

Molecular weight: 47351.84

Theoretical pI: 4.70

Amino acid composition: 
Ala (A)  25	  6.1%
Arg (R)  13	  3.2%
Asn (N)  17	  4.1%
Asp (D)  36	  8.7%
Cys (C)   1	  0.2%
Gln (Q)  17	  4.1%
Glu (E)  55	 13.3%
Gly (G)  18	  4.4%
His (H)  11	  2.7%
Ile (I)  21	  5.1%
Leu (L)  51	 12.4%
Lys (K)  41	 10.0%
Met (M)   3	  0.7%
Phe (F)  16	  3.9%
Pro (P)  10	  2.4%
Ser (S)  29	  7.0%
Thr (T)  13	  3.2%
Trp (W)   3	  0.7%
Tyr (Y)  13	  3.2%
Val (V)  19	  4.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 91
Total number of positively charged residues (Arg + Lys): 54

Atomic composition:

Carbon      C	      2101
Hydrogen    H	      3301
Nitrogen    N	       551
Oxygen      O	       684
Sulfur      S	         4

Formula: C2101H3301N551O684S4
Total number of atoms: 6641

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    35870
Abs 0.1% (=1 g/l)   0.758, assuming all pairs of Cys residues form cystines


Ext. coefficient    35870
Abs 0.1% (=1 g/l)   0.758, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.18
This classifies the protein as unstable.



Aliphatic index: 87.60

Grand average of hydropathicity (GRAVY): -0.728