ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAKRRQKKRT HAQLTPEQEQ GIPKSMVIRV GQTSLANHSL NQLVKDFRQI MQPHTAIKLK 

        70         80         90        100        110        120 
ERKSNKLKDF VVMCGPLGVT HLFMFTQSEK TGNVSLKIAR TPQGPTVTFQ VLDYSLGRDI 

       130        140        150        160        170        180 
KKFLKRPKSL NNDDVLNPPL LVLNGFSTSK RSGEDDQDVN VEKVIVSMFQ NIFPPLNPAR 

       190        200        210        220        230        240 
TSLNSIKRVF MINKDRETGE ISMRHYFIDI REVEISRNLK RLYKAKNNLS KTVPNLHRKE 

       250        260        270        280        290        300 
DISSLILDHD LGAYTSESEI EDDAIVRVVD NQDVKAKHSQ SLKSQRTPVE KKDNKEREKE 

       310        320        330        340        350        360 
TEEEDVEMEE PKPSENLQPT PRKKAIKLTE LGPRLTLKLV KIEEGICSGK VLHHEFVQKS 

       370        380        390        400        410        420 
SEEIKALEKR HAAKMRLKEQ RKKEQEENIA KKKAVKDAKK QRKLERRKAR AAEGGEGQGK 

       430        440        450 
DDAMSDDESS SSDSEHYGSV PEDLDSDLFS EVE 

References and documentation are available.

Number of amino acids: 453

Molecular weight: 51764.02

Theoretical pI: 9.28

Amino acid composition: 
Ala (A)  21	  4.6%
Arg (R)  30	  6.6%
Asn (N)  21	  4.6%
Asp (D)  29	  6.4%
Cys (C)   2	  0.4%
Gln (Q)  22	  4.9%
Glu (E)  44	  9.7%
Gly (G)  19	  4.2%
His (H)  12	  2.6%
Ile (I)  23	  5.1%
Leu (L)  39	  8.6%
Lys (K)  55	 12.1%
Met (M)  11	  2.4%
Phe (F)  13	  2.9%
Pro (P)  20	  4.4%
Ser (S)  37	  8.2%
Thr (T)  20	  4.4%
Trp (W)   0	  0.0%
Tyr (Y)   5	  1.1%
Val (V)  30	  6.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 73
Total number of positively charged residues (Arg + Lys): 85

Atomic composition:

Carbon      C	      2249
Hydrogen    H	      3711
Nitrogen    N	       665
Oxygen      O	       705
Sulfur      S	        13

Formula: C2249H3711N665O705S13
Total number of atoms: 7343

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     7575
Abs 0.1% (=1 g/l)   0.146, assuming all pairs of Cys residues form cystines


Ext. coefficient     7450
Abs 0.1% (=1 g/l)   0.144, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.72
This classifies the protein as unstable.



Aliphatic index: 77.22

Grand average of hydropathicity (GRAVY): -0.896