ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAKRVADAQI QRETYDSNES DDDVTPSTKV ASSAVMNRRK IAMPKRRMAF KPFGSAKSDE 

        70         80         90        100        110        120 
TKQASSFSFL NRADGTGEAQ VDNSPTTESN SRLKALNLQF KAKVDDLVLG KPLADLRPLF 

       130        140        150        160        170        180 
TRYELYIKNI LEAPVKSIEN PTQTKGNDAK PAKVEDVQKS SDSSSEDEVK VEGPKFTIDA 

       190        200        210        220        230        240 
KPPISDSVFS FGPKKENRKK DESDSENDIE IKGPEFKFSG TVSSDVFKLN PSTDKNEKKT 

       250        260        270        280        290        300 
ETNAKPFSFS SATSTTEQTK SKNPLSLTEA TKTNVDNNSK AEASFTFGTK HAADSQNNKP 

       310        320        330        340        350        360 
SFVFGQAAAK PSLEKSSFTF GSTTIEKKND ENSTSNSKPE KSSDSNDSNP SFSFSIPSKN 

       370        380        390        400        410        420 
TPDASKPSFS FGVPNSSKNE TSKPVFSFGA ATPSAKEASQ EDDNNNVEKP SSKPAFNLIS 

       430        440        450        460        470        480 
NAGTEKEKES KKDSKPAFSF GISNGSESKD SDKPSLPSAV DGENDKKEAT KPAFSFGINT 

       490        500        510        520        530        540 
NTTKTADTKA PTFTFGSSAL ADNKEDVKKP FSFGTSQPNN TPSFSFGKTT ANLPANSSTS 

       550        560        570        580        590        600 
PAPSIPSTGF KFSLPFEQKG SQTTTNDSKE ESTTEATGNE SQDATKVDAT PEESKPINLQ 

       610        620        630        640        650        660 
NGEEDEVALF SQKAKLMTFN AETKSYDSRG VGEMKLLKKK DDPSKVRLLC RSDGMGNVLL 

       670        680        690        700        710        720 
NATVVDSFKY EPLAPGNDNL IKAPTVAADG KLVTYIVKFK QKEEGRSFTK AIEDAKKEMK 

References and documentation are available.

Number of amino acids: 720

Molecular weight: 77880.84

Theoretical pI: 6.80

Amino acid composition: 
Ala (A)  59	  8.2%
Arg (R)  15	  2.1%
Asn (N)  51	  7.1%
Asp (D)  50	  6.9%
Cys (C)   1	  0.1%
Gln (Q)  18	  2.5%
Glu (E)  54	  7.5%
Gly (G)  33	  4.6%
His (H)   1	  0.1%
Ile (I)  19	  2.6%
Leu (L)  32	  4.4%
Lys (K)  89	 12.4%
Met (M)   8	  1.1%
Phe (F)  45	  6.2%
Pro (P)  48	  6.7%
Ser (S)  99	 13.8%
Thr (T)  60	  8.3%
Trp (W)   0	  0.0%
Tyr (Y)   6	  0.8%
Val (V)  32	  4.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 104
Total number of positively charged residues (Arg + Lys): 104

Atomic composition:

Carbon      C	      3382
Hydrogen    H	      5365
Nitrogen    N	       925
Oxygen      O	      1163
Sulfur      S	         9

Formula: C3382H5365N925O1163S9
Total number of atoms: 10844

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     8940
Abs 0.1% (=1 g/l)   0.115, assuming all pairs of Cys residues form cystines


Ext. coefficient     8940
Abs 0.1% (=1 g/l)   0.115, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.60
This classifies the protein as stable.



Aliphatic index: 48.71

Grand average of hydropathicity (GRAVY): -0.904