ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAKSVLVTGG AGYIGSHTVL QLLEGGYSAV VVDNYDNSSA ASLQRVKKLA GENGNRLSFH 

        70         80         90        100        110        120 
QVDLRDRPAL EKIFSETKFD AVIHFAGLKA VGESVEKPLL YYNNNIVGTV TLLEVMAQYG 

       130        140        150        160        170        180 
CKNLVFSSSA TVYGWPKEVP CTEESPISAT NPYGRTKLFI EEICRDVHRS DSEWKIILLR 

       190        200        210        220        230        240 
YFNPVGAHPS GYIGEDPLGV PNNLMPYVQQ VAVGRRPHLT VFGTDYKTKD GTGVRDYIHV 

       250        260        270        280        290        300 
MDLADGHIAA LRKLDDLKIS CEVYNLGTGN GTSVLEMVAA FEKASGKKIP LVMAGRRPGD 

       310        320        330        340        350 
AEVVYASTEK AERELNWKAK NGIEEMCRDL WNWASNNPYG YNSSSNGSSS 

References and documentation are available.

Number of amino acids: 350

Molecular weight: 38382.44

Theoretical pI: 6.27

Amino acid composition: 
Ala (A)  27	  7.7%
Arg (R)  16	  4.6%
Asn (N)  21	  6.0%
Asp (D)  17	  4.9%
Cys (C)   5	  1.4%
Gln (Q)   6	  1.7%
Glu (E)  23	  6.6%
Gly (G)  32	  9.1%
His (H)   8	  2.3%
Ile (I)  15	  4.3%
Leu (L)  30	  8.6%
Lys (K)  21	  6.0%
Met (M)   7	  2.0%
Phe (F)   9	  2.6%
Pro (P)  15	  4.3%
Ser (S)  28	  8.0%
Thr (T)  16	  4.6%
Trp (W)   5	  1.4%
Tyr (Y)  17	  4.9%
Val (V)  32	  9.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 40
Total number of positively charged residues (Arg + Lys): 37

Atomic composition:

Carbon      C	      1704
Hydrogen    H	      2665
Nitrogen    N	       467
Oxygen      O	       519
Sulfur      S	        12

Formula: C1704H2665N467O519S12
Total number of atoms: 5367

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    53080
Abs 0.1% (=1 g/l)   1.383, assuming all pairs of Cys residues form cystines


Ext. coefficient    52830
Abs 0.1% (=1 g/l)   1.376, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 32.45
This classifies the protein as stable.



Aliphatic index: 84.37

Grand average of hydropathicity (GRAVY): -0.273