ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MALAVTSSST AISGSSFSRS GASSESKALQ ICSIRLSDRT HLSQRRYSMK PLNAESHSRS 

        70         80         90        100        110        120 
ESWVTRASTL IAPEVEEKGG EVEDFEQLAK KLEDASPLEI MDKALERFGD QIAIAFSGAE 

       130        140        150        160        170        180 
DVALIEYARL TGKPFRVFSL DTGRLNPETY RLFDAVEKQY GIRIEYMFPD AVEVQALVRN 

       190        200        210        220        230        240 
KGLFSFYEDG HQECCRVRKV RPLRRALKGL KAWITGQRKD QSPGTRSEIP IVQVDPVFEG 

       250        260        270        280        290        300 
LDGGVGSLVK WNPLANVEGA DVWNFLRTMD VPVNALHAQG YVSIGCEPCT RPVLPGQHER 

       310        320        330        340        350        360 
EGRWWWEDAK AKECGLHKGN IKEEDGAADS KPAAVQEIFE SNNVVALSKG GVENLLKLEN 

       370        380        390        400        410        420 
RKEAWLVVLY APWCPFCQAM EASYIELAEK LAGKGVKVAK FRADGEQKEF AKQELQLGSF 

       430        440        450 
PTILLFPKRA PRAIKYPSEH RDVDSLMSFV NLLR 

References and documentation are available.

Number of amino acids: 454

Molecular weight: 50655.71

Theoretical pI: 6.45

Amino acid composition: 
Ala (A)  43	  9.5%
Arg (R)  31	  6.8%
Asn (N)  13	  2.9%
Asp (D)  21	  4.6%
Cys (C)   8	  1.8%
Gln (Q)  17	  3.7%
Glu (E)  42	  9.3%
Gly (G)  32	  7.0%
His (H)   7	  1.5%
Ile (I)  19	  4.2%
Leu (L)  42	  9.3%
Lys (K)  30	  6.6%
Met (M)   7	  1.5%
Phe (F)  19	  4.2%
Pro (P)  22	  4.8%
Ser (S)  36	  7.9%
Thr (T)  13	  2.9%
Trp (W)   9	  2.0%
Tyr (Y)  10	  2.2%
Val (V)  33	  7.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 63
Total number of positively charged residues (Arg + Lys): 61

Atomic composition:

Carbon      C	      2252
Hydrogen    H	      3554
Nitrogen    N	       630
Oxygen      O	       670
Sulfur      S	        15

Formula: C2252H3554N630O670S15
Total number of atoms: 7121

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    64900
Abs 0.1% (=1 g/l)   1.281, assuming all pairs of Cys residues form cystines


Ext. coefficient    64400
Abs 0.1% (=1 g/l)   1.271, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.83
This classifies the protein as unstable.



Aliphatic index: 82.95

Grand average of hydropathicity (GRAVY): -0.361