ProtParam
User-provided sequence:
10 20 30 40 50 60
MALDSVKHLP IHGVSAFSSV TVEIKSFFST VKPRKTSTFV YAFVVTFVAL TVFLAFSPSP
70 80 90 100 110 120
ITVALAPSIS SYVLPNITVS NSSNSSPSSL DSNFTTLRTP APENLTAVTK NLTFESPVAN
130 140 150 160 170 180
GTTDTNAKTI TIQFQTGHAK ENISCPDNKT ARDLDTHGAR KAPLSEVLAV NSTASPKRKQ
190 200 210 220 230 240
RRKSSLRKVI ESLKSCEFFE GDWVKDDSYP LYKPGSCNLI DEQFNCISNG RPDVDFQKLK
250 260 270 280 290 300
WKPKQCSLPR LNGGKLLEMI RGRRLVFVGD SLNRNMWESL VCILKGSVKD ESQVFEAHGR
310 320 330 340 350 360
HQFRWEAEYS FVFKDYNCTV EFFASPFLVQ EWEVTEKNGT KKETLRLDLV GKSSEQYKGA
370 380 390 400 410 420
DILVFNTGHW WTHEKTSKGE DYYQEGSTVH PKLDVDEAFR KALTTWGRWV DKNVNPKKSL
430 440 450 460 470 480
VFFRGYSPSH FSGGQWNAGG ACDDETEPIK NETYLTPYML KMEILERVLR GMKTPVTYLN
490 500 510 520 530 540
ITRLTDYRKD AHPSIYRKQK LSAEESKSPL LYQDCSHWCL PGVPDSWNEI FYAELLVKLD
550
QLGGKRRRKA LKDHRS
References and
documentation are available.
Number of amino acids: 556
Molecular weight: 63004.59
Theoretical pI: 9.06
Total number of negatively charged residues (Asp + Glu): 63
Total number of positively charged residues (Arg + Lys): 76
Atomic composition:
Carbon C 2825
Hydrogen H 4395
Nitrogen N 769
Oxygen O 835
Sulfur S 16
Formula: C
2825H
4395N
769O
835S
16
Total number of atoms: 8840
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 91955
Abs 0.1% (=1 g/l) 1.459, assuming all pairs of Cys residues form cystines
Ext. coefficient 91330
Abs 0.1% (=1 g/l) 1.450, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 45.16
This classifies the protein as unstable.
Aliphatic index: 73.78
Grand average of hydropathicity (GRAVY): -0.489