ProtParam
User-provided sequence:
10 20 30 40 50 60
MALEAMNTPT SSFTRIETKE DLMNDAVFIE PWLKRKRSKR QRSHSPSSSS SSPPRSRPKS
70 80 90 100 110 120
QNQDLTEEEY LALCLLMLAK DQPSQTRFHQ QSQSLTPPPE SKNLPYKCNV CEKAFPSYQA
130 140 150 160 170 180
LGGHKASHRI KPPTVISTTA DDSTAPTISI VAGEKHPIAA SGKIHECSIC HKVFPTGQAL
190 200 210 220 230 240
GGHKRCHYEG NLGGGGGGGS KSISHSGSVS STVSEERSHR GFIDLNLPAL PELSLHHNPI
250 260 270
VDEEILSPLT GKKPLLLTDH DQVIKKEDLS LKI
References and
documentation are available.
Number of amino acids: 273
Molecular weight: 29795.71
Theoretical pI: 8.67
Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 33
Atomic composition:
Carbon C 1296
Hydrogen H 2086
Nitrogen N 378
Oxygen O 407
Sulfur S 10
Formula: C
1296H
2086N
378O
407S
10
Total number of atoms: 4177
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 11835
Abs 0.1% (=1 g/l) 0.397, assuming all pairs of Cys residues form cystines
Ext. coefficient 11460
Abs 0.1% (=1 g/l) 0.385, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 72.68
This classifies the protein as unstable.
Aliphatic index: 73.63
Grand average of hydropathicity (GRAVY): -0.611