ProtParam
User-provided sequence:
10 20 30 40 50 60
MALEEEEESQ NAPFCVLDGL FCEEESEFHE QVDLCDESVE KFPFLNLGLS DHDMLWDDDE
70 80 90 100 110 120
LSTLISKQEP CLYDEILDDE FLVLCREKAL DWIFKVKSHY GFNSLTALLA VNYFDRFITS
130 140 150 160 170 180
RKFQTDKPWM SQLTALACLS LAAKVEEIRV PFLLDFQVEE ARYVFEAKTI QRMELLVLST
190 200 210 220 230 240
LDWRMHPVTP ISFFDHIIRR YSFKSHHQLE FLSRCESLLL SIIPDSRFLS FSPSVLATAI
250 260 270 280 290 300
MVSVIRDLKM CDEAVYQSQL MTLLKVDSEK VNKCYELVLD HSPSKKRMMN WMQQPASPIG
310 320 330 340 350 360
VFDASFSSDS SNESWVVSAS ASVSSSPSSE PLLKRRRVQE QQMRLSSINR MFFDVLSSSP
R
References and
documentation are available.
Number of amino acids: 361
Molecular weight: 41737.74
Theoretical pI: 4.84
Total number of negatively charged residues (Asp + Glu): 56
Total number of positively charged residues (Arg + Lys): 36
Atomic composition:
Carbon C 1866
Hydrogen H 2898
Nitrogen N 480
Oxygen O 561
Sulfur S 22
Formula: C
1866H
2898N
480O
561S
22
Total number of atoms: 5827
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 43930
Abs 0.1% (=1 g/l) 1.053, assuming all pairs of Cys residues form cystines
Ext. coefficient 43430
Abs 0.1% (=1 g/l) 1.041, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 63.66
This classifies the protein as unstable.
Aliphatic index: 89.89
Grand average of hydropathicity (GRAVY): -0.134