ProtParam
User-provided sequence:
10 20 30 40 50 60
MALEKHIEKV LFSDEVIAHR VNQLGIDITS DFSGDSEETP IFVGVATGAC LFLADLVRRI
70 80 90 100 110 120
DLPIAIDFIR AESYGSGTVS SGVPRVSFDL KLDITNKHVV LVEDIVDTGN TLSCLIEHMK
130 140 150 160 170 180
AKKASSVSVC TLLDKPSRRK VHYKLVGKGK FYSGFECPDE FVVGYGMDFA EQYRNLSYIG
VLKPEYYM
References and
documentation are available.
Number of amino acids: 188
Molecular weight: 20879.03
Theoretical pI: 5.54
Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 21
Atomic composition:
Carbon C 940
Hydrogen H 1481
Nitrogen N 241
Oxygen O 279
Sulfur S 8
Formula: C
940H
1481N
241O
279S
8
Total number of atoms: 2949
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 12170
Abs 0.1% (=1 g/l) 0.583, assuming all pairs of Cys residues form cystines
Ext. coefficient 11920
Abs 0.1% (=1 g/l) 0.571, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 39.44
This classifies the protein as stable.
Aliphatic index: 98.40
Grand average of hydropathicity (GRAVY): 0.064