ProtParam
User-provided sequence:
10 20 30 40 50 60
MALEPIDYTT HSREIDAEYL KIVRGSDPDT TWLIISPNAK KEYEPESTGS SFHDFLQLFD
70 80 90 100 110 120
ETKVQYGLAR VSPPGSDVEK IIIIGWCPDS APLKTRASFA ANFAAVANNL FKGYHVQVTA
130 140 150 160 170 180
RDEDDLDENE LLMKISNAAG ARYSIQTSSK QQGKASTPPV KKSFTPSKSP APVSKKEPVK
190 200 210 220 230 240
TPSPAPAAKI SSRVNDNNDD DDWNEPELKE RDFDQAPLKP NQSSYKPIGK IDLQKVIAEE
250 260 270 280 290 300
KAKEDPRLVQ KPTAAGSKID PSSDIANLKN ESKLKRDSEF NSFLGTTKPP SMTESSLKND
310 320 330 340 350 360
DDKVIKGFRN EKSPAQLWAE RKAKQNSGNA ETKAEAPKPE VPEDEPEGEP DVKDLKSKFE
370 380 390 400 410 420
GLAASEKEEE EMENKFAPPP KKSEPTIISP KPFSKPQEPV KAEEAEQPKT DYKKIGNPLP
430 440 450 460 470 480
GMHIEADNEE EPEENDDDWD DDEDEAAQPP LPSRNVASGA PVQKEEPEQE EIAPSLPSRN
490 500 510 520 530 540
SIPAPKQEEA PEQAPEEEIE EEAEEAAPQL PSRSSAAPPP PPRRATPEKK PKENPWATAE
550 560 570 580 590
YDYDAAEDNE LTFVENDKII NIEFVDDDWW LGELEKDGSK GLFPSNYVSL GN
References and
documentation are available.
Number of amino acids: 592
Molecular weight: 65576.22
Theoretical pI: 4.59
Total number of negatively charged residues (Asp + Glu): 122
Total number of positively charged residues (Arg + Lys): 76
Atomic composition:
Carbon C 2878
Hydrogen H 4488
Nitrogen N 768
Oxygen O 971
Sulfur S 6
Formula: C
2878H
4488N
768O
971S
6
Total number of atoms: 9111
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 60390
Abs 0.1% (=1 g/l) 0.921, assuming all pairs of Cys residues form cystines
Ext. coefficient 60390
Abs 0.1% (=1 g/l) 0.921, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 61.76
This classifies the protein as unstable.
Aliphatic index: 59.10
Grand average of hydropathicity (GRAVY): -1.042