ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MALFRALYII WVFLLIPLSN AEEFTPKVTR TLSRYVFDIV NFDDSNTLIR AEEDSVEISF 

        70         80         90        100        110        120 
DAGENWKTID EIEEPIESFV VDPFRGHDRA FAFVKTAPKF YVTDDQGKSW RPLTIPISEK 

       130        140        150        160        170        180 
ASNYFCDVTT HPIKKKHLII RCDLLTIKNS GLMYVGREIY TTNDGVSFSQ VKPSFGKIDG 

       190        200        210        220        230        240 
HISTARCDFI KSSEDSDLGG NDASILCLFR NTEYIESTGS TIDKSELILS ADGGETFKEL 

       250        260        270        280        290        300 
VQFKDKVVSR YEILKHHVIV LTQDDMYNEM SSTNIWISND VSTFQVARTP TKIRHVNMGQ 

       310        320        330        340        350        360 
IHEDSIGRIV LPVSRERDDE DSNQPGAAEV LISDSEGLKF LPINWIPNNQ FGYINVAYPG 

       370        380        390        400        410        420 
FLKGTFFGSF HPFIEYSDRK RKYSRQKVRE ETKVSVDNGL TWTNLKVVDR ENVDLFGCDV 

       430        440        450        460        470        480 
TKPERCSLQT HFYDLRNLNP SAGIMMISGI VGDGSAYNWK EEKTFISRDS GLTWRLVHNS 

       490        500        510        520        530        540 
TGLYTTGDLG NIIMYIPYRS NENGDVPSKF YYSLDQGKTW GEYDLIMPIY PYRLVSTISD 

       550        560        570        580        590        600 
GSGSKFILTG TSITEDPIFI TYSIDFSAVF DYKSCEEGDF EDWNLADGKC VNGAKYKYRR 

       610        620        630        640        650        660 
RKQDAQCLVK KAFKDLSLDE TPCNSCTGSD YECSFEFVRD AKGDCIPDYN LIALSDICDK 

       670        680        690        700        710        720 
SKGKSVLVKP LQLIKGDKCK TPMKIESVDI PCDEIPKEGS SDKEIVTTEN KFDFEIKFYQ 

       730        740        750        760        770        780 
YFDTVADESL VMLNSIGDAY ISHDGGQTIK RFDTDGEKIV EIVFNPYFNS SAYLFGSKGN 

       790        800        810        820        830        840 
IFLTHDRGYS FMIAKLPEAR QLGMPLDFSA KAQDTFIYYG GKNCESILSP ECHAVAYLTK 

       850        860        870        880        890        900 
DGGETFTEML DNAIHCEFAG TLFKYPSNDD MVMCQVKEKF SQTRSLVSST DFFQDDRKTV 

       910        920        930        940        950        960 
FENIIGYLST GGYIIVAVPH EDNELRAYVT NDGAEFTEAK FPYDEDIGKQ DAFTILGSEE 

       970        980        990       1000       1010       1020 
GSIFLHLATN LESGHDFGNL LKSNSNGTSF VTLEHAVNRN TFGYVDFEKV QGLEGIIITN 

      1030       1040       1050       1060       1070       1080 
IVSNSEKVGE NKEDEQLKTK ITFNDGSDWN FLKPPKKDSE GKKFPCDSVS LDKCSLHLHG 

      1090       1100       1110       1120       1130       1140 
YTERKDIRDT YSSGSALGMM FGVGNVGDRL LPYEECSTFL TTDGGETWTE VKKGPHQWEY 

      1150       1160       1170       1180       1190       1200 
GDHGGVLVLV PENAETDSIS YSTDFGKTWK DYKFCGDKVL VKDIITVPRD SALRFLLFGE 

      1210       1220       1230       1240       1250       1260 
AKNMGSGSFR TYTIDFRNIF ERQCEFDITG RKRADFKYSP LGSRTGCLFG HKTEFLRKTD 

      1270       1280       1290       1300       1310       1320 
EKCFIGNIPL SEFSRNVKNC PCTRQDFECD YNFYKASDGT CKLVKGLSSA NGADICKKEP 

      1330       1340       1350       1360       1370       1380 
DLIEYYDSSG YRKIPLSTCK GGLKLDAHLA PHPCPGKEKA FREKYSINTG AYALVFVTIL 

      1390       1400       1410       1420       1430       1440 
LVIFFVAWFV YDRGIRRNGG FSRFEEIRLG DDGLIENNRT DRVVNIIVRL GLCISLITKS 

      1450       1460       1470       1480       1490       1500 
AFQRAKAGTA QLSSKFRARF GNKKGATYSS LLHDQLSDEP DGFHEDSNDL SSFRGQGSNS 

      1510       1520       1530       1540 
EIEQEDVDTS QQEHTLRTDL LGASNIPDAL PARSASHESD LAAARSEDK 

References and documentation are available.

Number of amino acids: 1549

Molecular weight: 174427.94

Theoretical pI: 5.16

Amino acid composition: 
Ala (A)  70	  4.5%
Arg (R)  70	  4.5%
Asn (N)  72	  4.6%
Asp (D) 128	  8.3%
Cys (C)  35	  2.3%
Gln (Q)  34	  2.2%
Glu (E) 104	  6.7%
Gly (G) 117	  7.6%
His (H)  30	  1.9%
Ile (I) 106	  6.8%
Leu (L) 114	  7.4%
Lys (K) 110	  7.1%
Met (M)  19	  1.2%
Phe (F)  97	  6.3%
Pro (P)  54	  3.5%
Ser (S) 133	  8.6%
Thr (T)  97	  6.3%
Trp (W)  15	  1.0%
Tyr (Y)  61	  3.9%
Val (V)  83	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 232
Total number of positively charged residues (Arg + Lys): 180

Atomic composition:

Carbon      C	      7773
Hydrogen    H	     11952
Nitrogen    N	      2050
Oxygen      O	      2411
Sulfur      S	        54

Formula: C7773H11952N2050O2411S54
Total number of atoms: 24240

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   175515
Abs 0.1% (=1 g/l)   1.006, assuming all pairs of Cys residues form cystines


Ext. coefficient   173390
Abs 0.1% (=1 g/l)   0.994, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.84
This classifies the protein as stable.



Aliphatic index: 75.45

Grand average of hydropathicity (GRAVY): -0.415