ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MALKKVKGNF FERMFDKNLT DLVRGIRNNK DNEAKYISTC IEEIKQELRQ DNISVKCNAV 

        70         80         90        100        110        120 
AKLTYIQMLG YDISWAGFNI IEVMSSSRFT CKRIGYLAAS QCFHPDSELL MLTTNMIRKD 

       130        140        150        160        170        180 
LNSQNQYDAG VALSGLSCFI SPDLSRDLAN DIMTLMSSTK PYLRMKAVLM MYKVFLRYPE 

       190        200        210        220        230        240 
ALRPAFPKLK EKLEDPDPGV QSAAVNVICE LARKNPKNYL PLAPIFFKLM TTSTNNWMLI 

       250        260        270        280        290        300 
KIIKLFGALT PLEPRLGKKL IEPLTNLIHS TSAMSLLYEC INTVIAVLIS ISSGMPNHSA 

       310        320        330        340        350        360 
SIQLCVQKLR ILIEDSDQNL KYLGLLAMSK ILKTHPKSVQ AHKDLILACL DDKDESIRLR 

       370        380        390        400        410        420 
ALDLLYGMVS KKNLMEIVKR LLGHMERAEG SAYRDELLYK VIEICAQSSY LYVTNFEWYL 

       430        440        450        460        470        480 
TVLVELIQLE AGSRHGRLIA EQLLDVAIRV PVVRQFAVNE MTNLLDTFTV SAQSNSMYEV 

       490        500        510        520        530        540 
LYAAAWIVGE FAGELEDAEK TLNILLRPRL LPGHIQGVYV QNVIKLFARL ATTCLELQDL 

       550        560        570        580        590        600 
PGLVTLCDHV LDKLQHFNGS SDIEVQERAN SACMLIEMLR NQLSTSTDAM AMDTTTEGGI 

       610        620        630        640        650        660 
PPLAIEIVQE MTLLFTGELI PVAPKAQRKV PLPDGLDLDE WINAPPPEDA ASSSSSEHDK 

       670        680        690        700        710        720 
DELFVSATQA GTGADGGEKR RQSLELTPEQ LERQRMARLI EQSNNPHYLK STPTASGASN 

       730        740        750        760        770        780 
ADQYDNIDDI PITELPLDME GVAALRVGIT KRSDKYLQEQ QAAQGSKDGK KKHKKGKKSK 

       790        800        810        820        830        840 
KAKNKVAYNS SSESEGEPKP LHIVNTTLDM PEGVSMSDSE DKDGKYDPND PHRALDIELD 

       850        860        870        880        890        900 
ITEFEAPAVR SASKKSAADK ENLKTPADLA GGGNAAKKDR KKDKDKDKER KVKREHRESK 

       910        920        930        940        950        960 
RERKEAAVQP VIDLIDADTP TPSPSHISAT SNNNNTSTVL PDAPKHHKKK KNKEKTTDEA 

       970        980        990       1000       1010       1020 
PDALATATGS SIIDVGGEEA SEVASKVHKK KHKKEKSQRK EKKKASESAS VSAIVSIGDY 

      1030 
EQPLGISTPS KEIL 

References and documentation are available.

Number of amino acids: 1034

Molecular weight: 114845.79

Theoretical pI: 6.90

Amino acid composition: 
Ala (A)  87	  8.4%
Arg (R)  45	  4.4%
Asn (N)  47	  4.5%
Asp (D)  66	  6.4%
Cys (C)  13	  1.3%
Gln (Q)  37	  3.6%
Glu (E)  75	  7.3%
Gly (G)  48	  4.6%
His (H)  21	  2.0%
Ile (I)  64	  6.2%
Leu (L) 114	 11.0%
Lys (K)  94	  9.1%
Met (M)  30	  2.9%
Phe (F)  21	  2.0%
Pro (P)  50	  4.8%
Ser (S)  84	  8.1%
Thr (T)  53	  5.1%
Trp (W)   5	  0.5%
Tyr (Y)  26	  2.5%
Val (V)  54	  5.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 141
Total number of positively charged residues (Arg + Lys): 139

Atomic composition:

Carbon      C	      5048
Hydrogen    H	      8222
Nitrogen    N	      1394
Oxygen      O	      1564
Sulfur      S	        43

Formula: C5048H8222N1394O1564S43
Total number of atoms: 16271

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    66990
Abs 0.1% (=1 g/l)   0.583, assuming all pairs of Cys residues form cystines


Ext. coefficient    66240
Abs 0.1% (=1 g/l)   0.577, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.84
This classifies the protein as unstable.



Aliphatic index: 90.70

Grand average of hydropathicity (GRAVY): -0.399