ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MALKRIQKEL QDLGRDPPAQ CSAGPVGDDL FHWQATIMGP PESPYQGGVF FLTIHFPTDY 

        70         80         90        100        110        120 
PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS KVLLSICSLL CDPNPDDPLV 

       130        140 
PEIARIYKTD RERYNQLARE WTQKYAM 

References and documentation are available.

Number of amino acids: 147

Molecular weight: 16705.21

Theoretical pI: 6.81

Amino acid composition: 
Ala (A)   9	  6.1%
Arg (R)   8	  5.4%
Asn (N)   5	  3.4%
Asp (D)  10	  6.8%
Cys (C)   4	  2.7%
Gln (Q)   8	  5.4%
Glu (E)   5	  3.4%
Gly (G)   7	  4.8%
His (H)   3	  2.0%
Ile (I)  11	  7.5%
Leu (L)  14	  9.5%
Lys (K)   7	  4.8%
Met (M)   3	  2.0%
Phe (F)   6	  4.1%
Pro (P)  16	 10.9%
Ser (S)   9	  6.1%
Thr (T)   8	  5.4%
Trp (W)   3	  2.0%
Tyr (Y)   6	  4.1%
Val (V)   5	  3.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 15
Total number of positively charged residues (Arg + Lys): 15

Atomic composition:

Carbon      C	       756
Hydrogen    H	      1166
Nitrogen    N	       200
Oxygen      O	       214
Sulfur      S	         7

Formula: C756H1166N200O214S7
Total number of atoms: 2343

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    25690
Abs 0.1% (=1 g/l)   1.538, assuming all pairs of Cys residues form cystines


Ext. coefficient    25440
Abs 0.1% (=1 g/l)   1.523, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.49
This classifies the protein as unstable.



Aliphatic index: 82.31

Grand average of hydropathicity (GRAVY): -0.341