ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MALLSGNRKL DNYEPGCATL YLHDLGARPG PPAADVRPPA PRDLSARTEA LVSGPREIGG 

        70         80         90        100        110        120 
AGSGVTRLLP PPPSGASFLQ RGRPVTPALL QECGSRGRWG TNPLLCHFLL KWNQPYLICQ 

       130        140        150        160        170        180 
CETEAPHPAV PVHCPEHGVA ASPLCFFCSV TMSDRKAVIK NADMSEDMQQ DAVDCATQAM 

       190        200        210        220        230        240 
EKYNIEKDIA AYIKKEFDKK YNPTWHCIVG RNFGSYVTHE TKHFIYFYLG QVAILLFKSG 

References and documentation are available.

Number of amino acids: 240

Molecular weight: 26372.28

Theoretical pI: 8.02

Amino acid composition: 
Ala (A)  23	  9.6%
Arg (R)  13	  5.4%
Asn (N)   8	  3.3%
Asp (D)  11	  4.6%
Cys (C)  10	  4.2%
Gln (Q)   8	  3.3%
Glu (E)  12	  5.0%
Gly (G)  19	  7.9%
His (H)   8	  3.3%
Ile (I)   9	  3.8%
Leu (L)  22	  9.2%
Lys (K)  12	  5.0%
Met (M)   5	  2.1%
Phe (F)   9	  3.8%
Pro (P)  22	  9.2%
Ser (S)  13	  5.4%
Thr (T)  11	  4.6%
Trp (W)   3	  1.2%
Tyr (Y)   9	  3.8%
Val (V)  13	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 23
Total number of positively charged residues (Arg + Lys): 25

Atomic composition:

Carbon      C	      1175
Hydrogen    H	      1822
Nitrogen    N	       326
Oxygen      O	       336
Sulfur      S	        15

Formula: C1175H1822N326O336S15
Total number of atoms: 3674

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    30535
Abs 0.1% (=1 g/l)   1.158, assuming all pairs of Cys residues form cystines


Ext. coefficient    29910
Abs 0.1% (=1 g/l)   1.134, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.97
This classifies the protein as unstable.



Aliphatic index: 75.67

Grand average of hydropathicity (GRAVY): -0.262