ProtParam
User-provided sequence:
10 20 30 40 50 60
MALNDNPIPK SVPLHPKSGK YFHNLHARDL SNIYQQCYKQ IDETINQLVD STSPSTIGIE
70 80 90 100 110 120
EQVADITSTY KLLSTYESES NSFDEHIKDL KKNFKQSSDA CPQIDLSTWD KYRTGELTAP
130 140 150 160 170 180
KLSELYLNMP TPEPATMVNN TDTLKILKVL PYIWNDPTCV IPDLQNPADE DDLQIEGGKI
190 200 210 220 230 240
ELTCPITCKP YEAPLISRKC NHVFDRDGIQ NYLQGYTTRD CPQAACSQVV SMRDFVRDPI
250 260
MELRCKIAKM KESQEQDKRS SQAIDVL
References and
documentation are available.
Number of amino acids: 267
Molecular weight: 30354.34
Theoretical pI: 5.03
Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 29
Atomic composition:
Carbon C 1328
Hydrogen H 2104
Nitrogen N 356
Oxygen O 426
Sulfur S 15
Formula: C
1328H
2104N
356O
426S
15
Total number of atoms: 4229
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 27890
Abs 0.1% (=1 g/l) 0.919, assuming all pairs of Cys residues form cystines
Ext. coefficient 27390
Abs 0.1% (=1 g/l) 0.902, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 49.88
This classifies the protein as unstable.
Aliphatic index: 79.25
Grand average of hydropathicity (GRAVY): -0.605