ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MALNKLKNIP SLTNSSHSSI NGIASNAANS KPSGADTDDI DENDESGQSI LLNIISQLKP 

        70         80         90        100        110        120 
GCDLSRITLP TFILEKKSML ERITNQLQFP DVLLEAHSNK DGLQRFVKVV AWYLAGWHIG 

       130        140        150        160        170        180 
PRAVKKPLNP ILGEHFTAYW DLPNKQQAFY IAEQTSHHPP ESAYFYMIPE SNIRVDGVVV 

       190        200        210        220        230        240 
PKSKFLGNSS AAMMEGLTVL QFLDIKDANG KPEKYTLSQP NVYARGILFG KMRIELGDHM 

       250        260        270        280        290        300 
VIMGPKYQVD IEFKTKGFIS GTYDAIEGTI KDYDGKEYYQ ISGKWNDIMY IKDLREKSSK 

       310        320        330        340        350        360 
KTVLFDTHQH FPLAPKVRPL EEQGEYESRR LWKKVTDALA VRDHEVATEE KFQIENRQRE 

       370        380        390        400        410        420 
LAKKRAEDGV EFHSKLFRRA EPGEDLDYYI YKHIPEGTDK HEEQIRSILE TAPILPGQTF 

       430 
TEKFSIPAYK KHGIQKN 

References and documentation are available.

Number of amino acids: 437

Molecular weight: 49804.68

Theoretical pI: 6.72

Amino acid composition: 
Ala (A)  27	  6.2%
Arg (R)  18	  4.1%
Asn (N)  19	  4.3%
Asp (D)  26	  5.9%
Cys (C)   1	  0.2%
Gln (Q)  19	  4.3%
Glu (E)  34	  7.8%
Gly (G)  27	  6.2%
His (H)  14	  3.2%
Ile (I)  34	  7.8%
Leu (L)  36	  8.2%
Lys (K)  40	  9.2%
Met (M)   9	  2.1%
Phe (F)  18	  4.1%
Pro (P)  24	  5.5%
Ser (S)  29	  6.6%
Thr (T)  20	  4.6%
Trp (W)   5	  1.1%
Tyr (Y)  18	  4.1%
Val (V)  19	  4.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 60
Total number of positively charged residues (Arg + Lys): 58

Atomic composition:

Carbon      C	      2241
Hydrogen    H	      3500
Nitrogen    N	       602
Oxygen      O	       663
Sulfur      S	        10

Formula: C2241H3500N602O663S10
Total number of atoms: 7016

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    54320
Abs 0.1% (=1 g/l)   1.091, assuming all pairs of Cys residues form cystines


Ext. coefficient    54320
Abs 0.1% (=1 g/l)   1.091, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.58
This classifies the protein as unstable.



Aliphatic index: 81.26

Grand average of hydropathicity (GRAVY): -0.574