ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MALNLSHQLG VLAGTPIKSG EMTDSSLLSI SPPSARMMTP KAMNRNYKAH GTDPSPPMSP 

        70         80         90        100        110        120 
ILGATRADLS VACKAFAVEN GIGTIEEQRT YREGGIGGKK EGGGGVPVFV MMPLDSVTMG 

       130        140        150        160        170        180 
NTVNRRKAMK ASLQALKSAG VEGIMIDVWW GLVEKESPGT YNWGGYNELL ELAKKLGLKV 

       190        200        210        220        230        240 
QAVMSFHQCG GNVGDSVTIP LPQWVVEEVD KDPDLAYTDQ WGRRNHEYIS LGADTLPVLK 

       250        260        270        280        290        300 
GRTPVQCYAD FMRAFRDNFK HLLGETIVEI QVGMGPAGEL RYPSYPEQEG TWKFPGIGAF 

       310        320        330        340        350        360 
QCYDKYSLSS LKAAAETYGK PEWGSTGPTD AGHYNNWPED TQFFKKEGGG WNSEYGDFFL 

       370        380        390        400        410        420 
SWYSQMLLDH GERILSSAKS IFENMGVKIS VKIAGIHWHY GTRSHAPELT AGYYNTRFRD 

       430        440        450        460        470        480 
GYLPIAQMLA RHNAIFNFTC IEMRDHEQPQ DALCAPEKLV NQVALATLAA EVPLAGENAL 

       490        500        510        520        530        540 
PRYDDYAHEQ ILKASALNLD QNNEGEPREM CAFTYLRMNP ELFQADNWGK FVAFVKKMGE 

       550        560        570 
GRDSHRCREE VEREAEHFVH VTQPLVQEAA VALTH 

References and documentation are available.

Number of amino acids: 575

Molecular weight: 63762.22

Theoretical pI: 5.70

Amino acid composition: 
Ala (A)  51	  8.9%
Arg (R)  26	  4.5%
Asn (N)  25	  4.3%
Asp (D)  26	  4.5%
Cys (C)   8	  1.4%
Gln (Q)  22	  3.8%
Glu (E)  44	  7.7%
Gly (G)  55	  9.6%
His (H)  17	  3.0%
Ile (I)  22	  3.8%
Leu (L)  47	  8.2%
Lys (K)  30	  5.2%
Met (M)  21	  3.7%
Phe (F)  21	  3.7%
Pro (P)  32	  5.6%
Ser (S)  32	  5.6%
Thr (T)  28	  4.9%
Trp (W)  12	  2.1%
Tyr (Y)  22	  3.8%
Val (V)  34	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 70
Total number of positively charged residues (Arg + Lys): 56

Atomic composition:

Carbon      C	      2835
Hydrogen    H	      4358
Nitrogen    N	       776
Oxygen      O	       845
Sulfur      S	        29

Formula: C2835H4358N776O845S29
Total number of atoms: 8843

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    99280
Abs 0.1% (=1 g/l)   1.557, assuming all pairs of Cys residues form cystines


Ext. coefficient    98780
Abs 0.1% (=1 g/l)   1.549, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.64
This classifies the protein as stable.



Aliphatic index: 72.82

Grand average of hydropathicity (GRAVY): -0.391