ProtParam
User-provided sequence:
10 20 30 40 50 60
MALPSPASEE AEGPCQEANQ EYQEPVCSPV PEPEPEPEPE PEPEPEPVPV PPPEPQPEPE
70 80 90 100 110 120
PQPLPDPAPL PVLGFEAEPV QEPEPTPTVE TRGTARGFQP PEGGFGWIVV FAATWCNGSI
130 140 150 160 170 180
FGIHNSVGIL YSMLLEEEKE KNRQVEFQAA WVGALAMGMI FFCSPIVSIF TDRLGCRITA
190 200 210 220 230 240
TTGAAVAFIG LHTSSFTSPL SLRYFTYGIL FGCGCSFAFQ PSLVILDHYF QRRLGLANGV
250 260 270 280 290 300
VSAGSSIFSM SFPFLIKMLG DRIKLAQTFQ VLSTFMFVLT LLSLTYRPLL PSSQDTPSKR
310 320 330 340 350 360
GAHTLRQRFL VQFRKYFNMR VFRQRTYRIW AFGIAAAALG YFVPYVHLMK YVEDKFKEIK
370 380 390 400 410 420
ETWVLLVCIG ATSGLGRLVS GHISDSIPGL KKIYLQVLSF LLLGLMSMMI PLCRDFGGLI
430 440 450 460 470 480
VVCLFLGLCD GFFITIMAPI AFELVGPMQA SQAIGYLLGM MALPMIAGPP IAGLLRNCFG
490 500 510 520 530 540
NYHVAFYFAG VPPIIGAVIL FFVPLMHQRM FKKEQRESSK DKMLSHDPDP NGELLPGSPT
PEEPI
References and
documentation are available.
Number of amino acids: 545
Molecular weight: 60118.28
Theoretical pI: 5.52
Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 39
Atomic composition:
Carbon C 2769
Hydrogen H 4252
Nitrogen N 684
Oxygen O 748
Sulfur S 32
Formula: C
2769H
4252N
684O
748S
32
Total number of atoms: 8485
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 50600
Abs 0.1% (=1 g/l) 0.842, assuming all pairs of Cys residues form cystines
Ext. coefficient 49850
Abs 0.1% (=1 g/l) 0.829, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 50.57
This classifies the protein as unstable.
Aliphatic index: 90.72
Grand average of hydropathicity (GRAVY): 0.211