ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MALSSSLSCI KRYQVFSSFH GPDVRKGFLS HLHSVFASKG ITTFNDQKID RGQTIGPELI 

        70         80         90        100        110        120 
QGIREARVSI VVLSKKYASS SWCLDELVEI LKCKEALGQI VMTVFYEVDP SDVKKQSGVF 

       130        140        150        160        170        180 
GEAFEKTCQG KNEEVKIRWR NALAHVATIA GEHSLNWDNE AKMIQKIVTD VSDKLNLTPS 

       190        200        210        220        230        240 
RDFEGMVGME AHLKRLNSLL CLESDEVKMI GIWGPAGIGK TTIARTLFNK ISSIFPFKCF 

       250        260        270        280        290        300 
MENLKGSIKG GAEHYSKLSL QKQLLSEILK QENMKIHHLG TIKQWLHDQK VLIILDDVDD 

       310        320        330        340        350        360 
LEQLEVLAED PSWFGSGSRI IVTTEDKNIL KAHRIQDIYH VDFPSEEEAL EILCLSAFKQ 

       370        380        390        400        410        420 
SSIPDGFEEL ANKVAELCGN LPLGLCVVGA SLRRKSKNEW ERLLSRIESS LDKNIDNILR 

       430        440        450        460        470        480 
IGYDRLSTED QSLFLHIACF FNNEKVDYLT ALLADRKLDV VNGFNILADR SLVRISTDGH 

       490        500        510        520        530        540 
VVMHHYLLQK LGRRIVHEQW PNEPGKRQFL IEAEEIRDVL TKGTGTESVK GISFDTSNIE 

       550        560        570        580        590        600 
EVSVGKGAFE GMRNLQFLRI YRDSFNSEGT LQIPEDMEYI PPVRLLHWQN YPRKSLPQRF 

       610        620        630        640        650        660 
NPEHLVKIRM PSSKLKKLWG GIQPLPNLKS IDMSFSYSLK EIPNLSKATN LEILSLEFCK 

       670        680        690        700        710        720 
SLVELPFSIL NLHKLEILNV ENCSMLKVIP TNINLASLER LDMTGCSELR TFPDISSNIK 

       730        740        750        760        770        780 
KLNLGDTMIE DVPPSVGCWS RLDHLYIGSR SLKRLHVPPC ITSLVLWKSN IESIPESIIG 

       790        800        810        820        830        840 
LTRLDWLNVN SCRKLKSILG LPSSLQDLDA NDCVSLKRVC FSFHNPIRAL SFNNCLNLDE 

       850        860        870        880        890        900 
EARKGIIQQS VYRYICLPGK KIPEEFTHKA TGRSITIPLS PGTLSASSRF KASILILPVE 

       910        920        930        940        950        960 
SYETDDISCS LRTKGGVEVH CCELPYHFLL RSRSEHLFIF HGDLFPQGNK YHEVDVTMSE 

       970        980        990       1000       1010       1020 
ITFEFSHTKI GDKIIECGVQ IMTEGAEGDS SRELDSFETE SSSSQVDNFE TGGNNNHHTD 

      1030       1040       1050       1060 
GNGDGNYQAE GFKFFQDENI KTSKHTGFRS WLRELGLKVK KMNSHGVS 

References and documentation are available.

Number of amino acids: 1068

Molecular weight: 120555.22

Theoretical pI: 6.36

Amino acid composition: 
Ala (A)  38	  3.6%
Arg (R)  51	  4.8%
Asn (N)  53	  5.0%
Asp (D)  56	  5.2%
Cys (C)  24	  2.2%
Gln (Q)  33	  3.1%
Glu (E)  82	  7.7%
Gly (G)  68	  6.4%
His (H)  34	  3.2%
Ile (I)  83	  7.8%
Leu (L) 123	 11.5%
Lys (K)  76	  7.1%
Met (M)  19	  1.8%
Phe (F)  48	  4.5%
Pro (P)  39	  3.7%
Ser (S) 105	  9.8%
Thr (T)  44	  4.1%
Trp (W)  14	  1.3%
Tyr (Y)  19	  1.8%
Val (V)  59	  5.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 138
Total number of positively charged residues (Arg + Lys): 127

Atomic composition:

Carbon      C	      5368
Hydrogen    H	      8531
Nitrogen    N	      1465
Oxygen      O	      1599
Sulfur      S	        43

Formula: C5368H8531N1465O1599S43
Total number of atoms: 17006

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   106810
Abs 0.1% (=1 g/l)   0.886, assuming all pairs of Cys residues form cystines


Ext. coefficient   105310
Abs 0.1% (=1 g/l)   0.874, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.13
This classifies the protein as unstable.



Aliphatic index: 94.80

Grand average of hydropathicity (GRAVY): -0.255