ProtParam
User-provided sequence:
10 20 30 40 50 60
MALVMHTYKG NKGANKALIA AEYAGVKIEE SADFQMGVTN KSPEFLKMNP IGKVPVLETP
70 80 90 100 110 120
EGPIFESNAI ARYVSRKNGD NSLNGSSLIE YAHIEQWIDF SSLEIDANML KWFAPRMGYA
130 140 150 160 170 180
PFSAPAEEAA ISALKRGLEA LNTHLASNTF LVGHSVTLAD IVTICNLNLG FATVMTKKFT
190 200 210 220 230 240
SAFPHVERYF WTMVNQPEFK KVLGDAKQTE AVPPVPTKKA PQPAKPKEEP KKAAPVAEAP
250 260 270 280 290 300
KPAEEEEAPK PKAKNPLDLL PPSPMVLDDW KRLYSNTKSN FREVAIKGFW DMYDPEGYSL
310 320 330 340 350 360
WFCDYKYNDE NMVSFVTLNK VGGFLQRMDL ARKYSFGKML ICGSEGPFKV KGLWLFRGPE
370 380 390 400 410
IPKFIMDEVY DMELYEWTKV DISDEAQKER VSQMIEDAEP FEGEALLDAK CFK
References and
documentation are available.
Number of amino acids: 413
Molecular weight: 46400.34
Theoretical pI: 5.55
Total number of negatively charged residues (Asp + Glu): 57
Total number of positively charged residues (Arg + Lys): 50
Atomic composition:
Carbon C 2107
Hydrogen H 3245
Nitrogen N 531
Oxygen O 609
Sulfur S 20
Formula: C
2107H
3245N
531O
609S
20
Total number of atoms: 6512
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 65110
Abs 0.1% (=1 g/l) 1.403, assuming all pairs of Cys residues form cystines
Ext. coefficient 64860
Abs 0.1% (=1 g/l) 1.398, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.66
This classifies the protein as unstable.
Aliphatic index: 74.46
Grand average of hydropathicity (GRAVY): -0.350