ProtParam
User-provided sequence:
10 20 30 40 50 60
MALVSRRSTR SESTSITKEE HTGEGSLTKL FFRWLVTLEG DQDINDGKGY ISLPNVSNYI
70 80 90 100 110 120
FFLGGRFRTV KGAKPLWLGV LLAIVCPMVL FSIFEAHKLW HTQNGYKVLV IFFYYFWVIT
130 140 150 160 170 180
LASFIRTATS DPGVLPRNIH LSQLRNNYQI PQEYYNLITL PTHSSISKDI TIKYCPSCRI
190 200 210 220 230 240
WRPPRSSHCS TCNVCVMVHD HHCIWVNNCI GKRNYRFFLI FLLGAILSSV ILLTNCAIHI
250 260 270 280 290 300
ARESGGPRDC PVAILLLCYA GLTLWYPAIL FTYHIFMAGN QQTTREFLKG IGSKKNPVFH
310 320 330 340 350
RVVKEENIYN KGSFLKNMGH LMLEPRGPSF VSARKPHEAG DWRFMDLSPA HSFEKIQKI
References and
documentation are available.
Number of amino acids: 359
Molecular weight: 41098.95
Theoretical pI: 9.48
Total number of negatively charged residues (Asp + Glu): 23
Total number of positively charged residues (Arg + Lys): 40
Atomic composition:
Carbon C 1883
Hydrogen H 2905
Nitrogen N 505
Oxygen O 494
Sulfur S 18
Formula: C
1883H
2905N
505O
494S
18
Total number of atoms: 5805
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 65485
Abs 0.1% (=1 g/l) 1.593, assuming all pairs of Cys residues form cystines
Ext. coefficient 64860
Abs 0.1% (=1 g/l) 1.578, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.08
This classifies the protein as unstable.
Aliphatic index: 94.21
Grand average of hydropathicity (GRAVY): 0.020