ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAMAVFRREG RRLLPSIAAR PIAAIRSPLS SDQEEGLLGV RSISTQVVRN RMKSVKNIQK 

        70         80         90        100        110        120 
ITKAMKMVAA SKLRAVQGRA ENSRGLWQPF TALLGDNPSI DVKKSVVVTL SSDKGLCGGI 

       130        140        150        160        170        180 
NSTVVKVSRA LYKLNAGPEK EVQFVIVGEK AKAIMFRDSK NDIVLSVTEL NKNPLNYAQV 

       190        200        210        220        230        240 
SVLADDILKN VEFDALRIVY NKFHSVVAFL PTVSTVLSPE IIEKESEIGG KLGELDSYEI 

       250        260        270        280        290        300 
EGGETKGEIL QNLAEFQFSC VMFNAVLENA CSEMGARMSA MDSSSRNAGE MLDRLTLTYN 

       310        320 
RTRQASITTE LIEIISGASA LEAAK 

References and documentation are available.

Number of amino acids: 325

Molecular weight: 35447.95

Theoretical pI: 9.01

Amino acid composition: 
Ala (A)  31	  9.5%
Arg (R)  20	  6.2%
Asn (N)  17	  5.2%
Asp (D)  12	  3.7%
Cys (C)   3	  0.9%
Gln (Q)  10	  3.1%
Glu (E)  25	  7.7%
Gly (G)  20	  6.2%
His (H)   1	  0.3%
Ile (I)  22	  6.8%
Leu (L)  31	  9.5%
Lys (K)  22	  6.8%
Met (M)  11	  3.4%
Phe (F)  10	  3.1%
Pro (P)   9	  2.8%
Ser (S)  32	  9.8%
Thr (T)  14	  4.3%
Trp (W)   1	  0.3%
Tyr (Y)   5	  1.5%
Val (V)  29	  8.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 42

Atomic composition:

Carbon      C	      1552
Hydrogen    H	      2569
Nitrogen    N	       437
Oxygen      O	       478
Sulfur      S	        14

Formula: C1552H2569N437O478S14
Total number of atoms: 5050

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    13075
Abs 0.1% (=1 g/l)   0.369, assuming all pairs of Cys residues form cystines


Ext. coefficient    12950
Abs 0.1% (=1 g/l)   0.365, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.43
This classifies the protein as unstable.



Aliphatic index: 99.02

Grand average of hydropathicity (GRAVY): -0.054