ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAMKAVCVLK GDGPVQGVIH FEQKASGEPV VVSGQITGLT EGEHGFHVHQ YGDNTQGCTT 

        70         80         90        100        110        120 
AGPHFNPHSK KHGGPADEER HVGDLGNVAA GKDGVANVSI EDRVISLSGE HSIIGRTMVV 

       130        140        150 
HEKQDDLGKG GNEESTKTGN AGSRLACGVI GIAQ 

References and documentation are available.

Number of amino acids: 154

Molecular weight: 15911.66

Theoretical pI: 5.88

Amino acid composition: 
Ala (A)  11	  7.1%
Arg (R)   4	  2.6%
Asn (N)   6	  3.9%
Asp (D)   8	  5.2%
Cys (C)   3	  1.9%
Gln (Q)   7	  4.5%
Glu (E)  11	  7.1%
Gly (G)  26	 16.9%
His (H)  10	  6.5%
Ile (I)   8	  5.2%
Leu (L)   6	  3.9%
Lys (K)   9	  5.8%
Met (M)   3	  1.9%
Phe (F)   3	  1.9%
Pro (P)   5	  3.2%
Ser (S)   9	  5.8%
Thr (T)   8	  5.2%
Trp (W)   0	  0.0%
Tyr (Y)   1	  0.6%
Val (V)  16	 10.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 19
Total number of positively charged residues (Arg + Lys): 13

Atomic composition:

Carbon      C	       677
Hydrogen    H	      1082
Nitrogen    N	       208
Oxygen      O	       224
Sulfur      S	         6

Formula: C677H1082N208O224S6
Total number of atoms: 2197

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     1615
Abs 0.1% (=1 g/l)   0.101, assuming all pairs of Cys residues form cystines


Ext. coefficient     1490
Abs 0.1% (=1 g/l)   0.094, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 24.94
This classifies the protein as stable.



Aliphatic index: 72.73

Grand average of hydropathicity (GRAVY): -0.404