ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAMQKQQLTV VVRVRPLNSG EKSRKAFQCV FPLDKKVPRT SLSKKLNLFS FKRVLLVDPE 

        70         80         90        100        110        120 
KFENNILRQN RQHERKFEFD ASFGATSNQE DVHETTTGPI IDSVVEGFNA TVFAYGATGS 

       130        140        150        160        170        180 
GKTFTMIGTK DRPGLMTLMT KTLYEKLDNQ YQVLLSYMEI YNEIIRDLLN PSGGDLELLE 

       190        200        210        220        230        240 
DERGNIRVPE TTKFVNYRDS KLTRLLKDSL GGTAKTCMIA HVTPSSSNFE ETYNTLMYAS 

       250        260        270        280        290        300 
RAMNITNKPM RNRPASADQV YAEAMNAIRK EVGSQIKNSS SITGLAARSS VQERSSSRNR 

       310        320        330        340        350        360 
PKSISRNSSL TRAAQPMPID RGRGGSLFTQ LKEQYMTLSD KQKRLREKLM ATNQEAYSLE 

       370        380        390 
MSLISKNAII NAWEKHHHDK TAESIERLKK DGCGTI 

References and documentation are available.

Number of amino acids: 396

Molecular weight: 44718.99

Theoretical pI: 9.62

Amino acid composition: 
Ala (A)  25	  6.3%
Arg (R)  29	  7.3%
Asn (N)  24	  6.1%
Asp (D)  17	  4.3%
Cys (C)   3	  0.8%
Gln (Q)  17	  4.3%
Glu (E)  27	  6.8%
Gly (G)  21	  5.3%
His (H)   6	  1.5%
Ile (I)  20	  5.1%
Leu (L)  35	  8.8%
Lys (K)  31	  7.8%
Met (M)  15	  3.8%
Phe (F)  14	  3.5%
Pro (P)  14	  3.5%
Ser (S)  37	  9.3%
Thr (T)  30	  7.6%
Trp (W)   1	  0.3%
Tyr (Y)  11	  2.8%
Val (V)  19	  4.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 44
Total number of positively charged residues (Arg + Lys): 60

Atomic composition:

Carbon      C	      1943
Hydrogen    H	      3160
Nitrogen    N	       568
Oxygen      O	       604
Sulfur      S	        18

Formula: C1943H3160N568O604S18
Total number of atoms: 6293

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    22015
Abs 0.1% (=1 g/l)   0.492, assuming all pairs of Cys residues form cystines


Ext. coefficient    21890
Abs 0.1% (=1 g/l)   0.490, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.11
This classifies the protein as stable.



Aliphatic index: 74.39

Grand average of hydropathicity (GRAVY): -0.610