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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAMTAAAVPS SGSFQKQDEE WRAVLSPEQF RVLRLKGTDK RGKGEFTKKF EEGTYSCAGC

70 80 90 100 110 120
GTALYKSTTK FDSGCGWPAF FDAIPGAIKQ TPEAGGRRME ITCAVCDGHL GHVFKGEGYS

130 140
TPTDQRHCVN SVSLKFASAD SSK


References and documentation are available.

Number of amino acids: 143 Molecular weight: 15429.38 Theoretical pI: 8.54
Amino acid composition: 
Ala (A) 14 9.8% Arg (R) 7 4.9% Asn (N) 1 0.7% Asp (D) 7 4.9% Cys (C) 6 4.2% Gln (Q) 5 3.5% Glu (E) 9 6.3% Gly (G) 16 11.2% His (H) 3 2.1% Ile (I) 3 2.1% Leu (L) 6 4.2% Lys (K) 12 8.4% Met (M) 3 2.1% Phe (F) 9 6.3% Pro (P) 6 4.2% Ser (S) 13 9.1% Thr (T) 11 7.7% Trp (W) 2 1.4% Tyr (Y) 3 2.1% Val (V) 7 4.9% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 16 Total number of positively charged residues (Arg + Lys): 19 Atomic composition: Carbon C 673 Hydrogen H 1044 Nitrogen N 190 Oxygen O 209 Sulfur S 9 Formula: C673H1044N190O209S9 Total number of atoms: 2125 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 15845 Abs 0.1% (=1 g/l) 1.027, assuming all pairs of Cys residues form cystines Ext. coefficient 15470 Abs 0.1% (=1 g/l) 1.003, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 40.17 This classifies the protein as unstable. Aliphatic index: 48.53 Grand average of hydropathicity (GRAVY): -0.475