ProtParam
User-provided sequence:
10 20 30 40 50 60
MAMTAAAVPS SGSFQKQDEE WRAVLSPEQF RVLRLKGTDK RGKGEFTKKF EEGTYSCAGC
70 80 90 100 110 120
GTALYKSTTK FDSGCGWPAF FDAIPGAIKQ TPEAGGRRME ITCAVCDGHL GHVFKGEGYS
130 140
TPTDQRHCVN SVSLKFASAD SSK
References and
documentation are available.
Number of amino acids: 143
Molecular weight: 15429.38
Theoretical pI: 8.54
Total number of negatively charged residues (Asp + Glu): 16
Total number of positively charged residues (Arg + Lys): 19
Atomic composition:
Carbon C 673
Hydrogen H 1044
Nitrogen N 190
Oxygen O 209
Sulfur S 9
Formula: C
673H
1044N
190O
209S
9
Total number of atoms: 2125
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 15845
Abs 0.1% (=1 g/l) 1.027, assuming all pairs of Cys residues form cystines
Ext. coefficient 15470
Abs 0.1% (=1 g/l) 1.003, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 40.17
This classifies the protein as unstable.
Aliphatic index: 48.53
Grand average of hydropathicity (GRAVY): -0.475