ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAMYVIYESS SGYGLFEVHG LDEIGQNTEA VRTSVSDLSR FGRVVQLTAF HPFESALDAL 

        70         80         90        100        110        120 
NQVNAVSEGV MTDELRSFLE LNLPKVKEGK KPKFSLGLAE PKLGSHIFEA TKIPCQSNEF 

       130        140        150        160        170        180 
VLELLRGVRQ HFDRFIKDLK PGDLEKSQLG LAHSYSRAKV KFNVNRVDNM VIQAIFMLDT 

       190        200        210        220        230        240 
LDKDINSFAM RVREWYSWHF PELVKIVNDN YLYARVSKMI DDKSKLTEDH IPMLTEVLGD 

       250        260        270        280        290        300 
EDKAKEVIEA GKASMGSDLS PLDLINVQTF AQKVMDLADY RKKLYDYLVT KMSDIAPNLA 

       310        320        330        340        350        360 
ALIGEMVGAR LISHAGSLTN LAKCPSSTLQ ILGAEKALFR ALKTRGNTPK YGLIFHSSFI 

       370        380        390        400        410        420 
GRASAKNKGR IARYLANKCS IASRIDCFAD GATTAFGEKL REQVEERLEF YDKGVAPRKN 

       430        440        450        460        470        480 
VDVMKEVIEN LKQEEEGKEP VDASVKKSKK KKAKGEEEEE VVAMEEDKSE KKKKKEKRKM 

       490        500        510        520 
ETAEENEKSE KKKTKKSKAG GEEETDDGHS TKKKKKKSKS AE 

References and documentation are available.

Number of amino acids: 522

Molecular weight: 58673.26

Theoretical pI: 8.78

Amino acid composition: 
Ala (A)  42	  8.0%
Arg (R)  24	  4.6%
Asn (N)  20	  3.8%
Asp (D)  31	  5.9%
Cys (C)   4	  0.8%
Gln (Q)  12	  2.3%
Glu (E)  52	 10.0%
Gly (G)  31	  5.9%
His (H)  10	  1.9%
Ile (I)  23	  4.4%
Leu (L)  47	  9.0%
Lys (K)  66	 12.6%
Met (M)  15	  2.9%
Phe (F)  21	  4.0%
Pro (P)  14	  2.7%
Ser (S)  40	  7.7%
Thr (T)  20	  3.8%
Trp (W)   2	  0.4%
Tyr (Y)  13	  2.5%
Val (V)  35	  6.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 83
Total number of positively charged residues (Arg + Lys): 90

Atomic composition:

Carbon      C	      2592
Hydrogen    H	      4194
Nitrogen    N	       714
Oxygen      O	       794
Sulfur      S	        19

Formula: C2592H4194N714O794S19
Total number of atoms: 8313

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    30620
Abs 0.1% (=1 g/l)   0.522, assuming all pairs of Cys residues form cystines


Ext. coefficient    30370
Abs 0.1% (=1 g/l)   0.518, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.68
This classifies the protein as stable.



Aliphatic index: 79.79

Grand average of hydropathicity (GRAVY): -0.570