ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MANDAAACAE RATNDMLIGP DWAINIELCD LINMDPSQAK EAVKVLKKRL GSKNSKVQIL 

        70         80         90        100        110        120 
ALYALETLSK NCGENVYQLI IDRGLLNDMV KIVKKKPELN VREKILTLLD TWQEAFGGRG 

       130        140        150        160        170        180 
GRYPQYYNAY NDLRSAGIEF PPRTESSLSF FTPPQTQPDE DAAIQASLQG DDASSLSLEE 

       190        200        210        220        230        240 
IQSAEGSVDV LMDMLGAHDP GNPESLKEEV IVDLVEQCRT YQRRVMTLVN TTTDEELLCQ 

       250        260        270        280        290        300 
GLALNDNLQH VLQRHDDIAN VGSVPSNGRN TRAPPPVQIV DINHDDEDDE SDDEFARLAH 

       310        320        330        340        350        360 
RSSTPTRRPV HGSDSGMVDI LSGDVYKPQG NSSSQGVKKP PPPPPHTSSS SSSPVFDDAS 

       370        380        390        400        410        420 
PQQSKSSEVI RNLPPPPSRH NQRQQFFEHH HSSSGSDSSY EGQTRNLSLT SSEPQKEEKP 

       430        440 
EDLLFKDLVE FAKTRSSKAN NNNRSL 

References and documentation are available.

Number of amino acids: 446

Molecular weight: 49254.56

Theoretical pI: 4.97

Amino acid composition: 
Ala (A)  28	  6.3%
Arg (R)  24	  5.4%
Asn (N)  27	  6.1%
Asp (D)  36	  8.1%
Cys (C)   5	  1.1%
Gln (Q)  25	  5.6%
Glu (E)  31	  7.0%
Gly (G)  23	  5.2%
His (H)  11	  2.5%
Ile (I)  18	  4.0%
Leu (L)  41	  9.2%
Lys (K)  22	  4.9%
Met (M)   8	  1.8%
Phe (F)  10	  2.2%
Pro (P)  32	  7.2%
Ser (S)  49	 11.0%
Thr (T)  19	  4.3%
Trp (W)   2	  0.4%
Tyr (Y)   9	  2.0%
Val (V)  26	  5.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 67
Total number of positively charged residues (Arg + Lys): 46

Atomic composition:

Carbon      C	      2119
Hydrogen    H	      3372
Nitrogen    N	       616
Oxygen      O	       710
Sulfur      S	        13

Formula: C2119H3372N616O710S13
Total number of atoms: 6830

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    24660
Abs 0.1% (=1 g/l)   0.501, assuming all pairs of Cys residues form cystines


Ext. coefficient    24410
Abs 0.1% (=1 g/l)   0.496, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 55.05
This classifies the protein as unstable.



Aliphatic index: 74.78

Grand average of hydropathicity (GRAVY): -0.717