ProtParam
User-provided sequence:
10 20 30 40 50 60
MANDGIQRND NRKGFKTVQF SAYSKEIDVI MKKISFLERN ITQQLDTLPH FPKTLPPNHK
70 80 90 100 110 120
DCVSRKHRAR RGWSSQLKNL LGIYSKEEIF TLDNLAATLH DQVLKLQATL FPNAILKQVH
130 140 150 160 170 180
LDNANIENKR ILKEITYKYL SNENCKEENK FGTFIVKRIF FGDLSLGVSV LINRIAFESA
190 200 210 220 230
TSSIMVVRSS FIESDFFYED YLIFDCRAKR RKKLKRKILF ISTTMNFNYQ TKV
References and
documentation are available.
Number of amino acids: 233
Molecular weight: 27228.56
Theoretical pI: 9.76
Total number of negatively charged residues (Asp + Glu): 24
Total number of positively charged residues (Arg + Lys): 39
Atomic composition:
Carbon C 1229
Hydrogen H 1959
Nitrogen N 339
Oxygen O 345
Sulfur S 7
Formula: C
1229H
1959N
339O
345S
7
Total number of atoms: 3879
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 16055
Abs 0.1% (=1 g/l) 0.590, assuming all pairs of Cys residues form cystines
Ext. coefficient 15930
Abs 0.1% (=1 g/l) 0.585, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 48.55
This classifies the protein as unstable.
Aliphatic index: 90.39
Grand average of hydropathicity (GRAVY): -0.384