ProtParam
User-provided sequence:
10 20 30 40 50 60
MANDGNGDNN DDPLRQYLMN PRINPPPPSL LTLPENNDVT IPMPITPLEL KNRLIFGSFV
70 80 90 100 110 120
RSRKESSLPI DALSQNPSTS SSATTSFSDS TDLLLPLTEP NKPVRKSKPT INFHRSKTAP
130 140 150 160 170 180
AMAAINNISH PNDPKTDQQS DSKTIVNQAV ALLVVYLSLG VLIYWLNRDS YNVKQTHPVV
190 200 210 220 230 240
DALYFCIVTM CTIGYGDITP DSVVTKLFSI FFVLVGFGFM DILLSGMVTY VLDLQENYML
250 260 270 280 290 300
ETARNESLNL NDRDKVRSYI IDVKKGRMRI RLKVGLALGV VVLCLGFGVL IMHFVEKIGW
310 320 330 340 350 360
LDSFYFSVMS VTTVGYGDRA FNTLAGRLLA AMWLLVSTLA VARAILFLAE SRVDKRNRER
370 380 390 400 410 420
AKKVLGESMS ISQFLDADID CNGCVSKAEF VIYKLKKMDK ITEKDINPIG FQFDKLDRTN
430 440
SGRITLLDLL ESSTKDLPTA TSI
References and
documentation are available.
Number of amino acids: 443
Molecular weight: 49326.13
Theoretical pI: 8.43
Total number of negatively charged residues (Asp + Glu): 46
Total number of positively charged residues (Arg + Lys): 49
Atomic composition:
Carbon C 2209
Hydrogen H 3547
Nitrogen N 585
Oxygen O 651
Sulfur S 19
Formula: C
2209H
3547N
585O
651S
19
Total number of atoms: 7011
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 34630
Abs 0.1% (=1 g/l) 0.702, assuming all pairs of Cys residues form cystines
Ext. coefficient 34380
Abs 0.1% (=1 g/l) 0.697, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.53
This classifies the protein as stable.
Aliphatic index: 101.40
Grand average of hydropathicity (GRAVY): 0.004