ProtParam
User-provided sequence:
10 20 30 40 50 60
MANEGSDPLL QYMISPRLKK PPQLLFPLPE DNEVAIPMPM TPSEFKERLI FGPFSCSPRD
70 80 90 100 110 120
SSHFIDSMKQ PSPSSSSTAV NNPFSDSSTL DPLLPPPPPQ PEPWLSDQTS SHCQGHALHR
130 140 150 160 170 180
SKTAPAMAVI NDLHHPIRQK DPTETSRSVV RQAFALLVVY LSLGVLIYWL NRDHYVVNQT
190 200 210 220 230 240
HPVVDGLYFC IVTMCTIGYG DITPNSVVTK LFSIMFVLVG FGFIDILLSG MVSYVLDLQE
250 260 270 280 290 300
SYMLDSAKRR DEPEKRRSYI IDVKKGRMRI RLKVALALGV VVLCIAVGVG IMHFIEEIGW
310 320 330 340 350 360
LDSFYLSVMS VTTVGYGDRA FKTLPGRLFA AIWLLVSTLA VARAFLYLAE ARVDKRNRER
370 380 390 400 410 420
AKKVLCETMS VSQFFAADID NNGCVSKAEY VIYKLKEMEK ITDKDILPIS KQFDKLDRCS
430
NGKITLLDLL EGGSGD
References and
documentation are available.
Number of amino acids: 436
Molecular weight: 48731.63
Theoretical pI: 7.10
Total number of negatively charged residues (Asp + Glu): 47
Total number of positively charged residues (Arg + Lys): 47
Atomic composition:
Carbon C 2197
Hydrogen H 3477
Nitrogen N 575
Oxygen O 628
Sulfur S 23
Formula: C
2197H
3477N
575O
628S
23
Total number of atoms: 6900
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 43360
Abs 0.1% (=1 g/l) 0.890, assuming all pairs of Cys residues form cystines
Ext. coefficient 42860
Abs 0.1% (=1 g/l) 0.880, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 48.14
This classifies the protein as unstable.
Aliphatic index: 96.77
Grand average of hydropathicity (GRAVY): 0.014