ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MANLMMRLPI SLRSFSVSAS SSNGSPPVIG GSSGGVGPMI VELPLEKIRR PLMRTRSNDQ 

        70         80         90        100        110        120 
NKVKELMDSI RQIGLQVPID VIEVDGTYYG FSGCHRYEAH QKLGLPTIRC KIRKGTKETL 


RHHLR 

References and documentation are available.

Number of amino acids: 125

Molecular weight: 13914.25

Theoretical pI: 10.17

Amino acid composition: 
Ala (A)   3	  2.4%
Arg (R)  12	  9.6%
Asn (N)   4	  3.2%
Asp (D)   4	  3.2%
Cys (C)   2	  1.6%
Gln (Q)   4	  3.2%
Glu (E)   6	  4.8%
Gly (G)  12	  9.6%
His (H)   4	  3.2%
Ile (I)  10	  8.0%
Leu (L)  12	  9.6%
Lys (K)   7	  5.6%
Met (M)   6	  4.8%
Phe (F)   2	  1.6%
Pro (P)   8	  6.4%
Ser (S)  13	 10.4%
Thr (T)   5	  4.0%
Trp (W)   0	  0.0%
Tyr (Y)   3	  2.4%
Val (V)   8	  6.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 10
Total number of positively charged residues (Arg + Lys): 19

Atomic composition:

Carbon      C	       605
Hydrogen    H	      1006
Nitrogen    N	       184
Oxygen      O	       175
Sulfur      S	         8

Formula: C605H1006N184O175S8
Total number of atoms: 1978

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     4595
Abs 0.1% (=1 g/l)   0.330, assuming all pairs of Cys residues form cystines


Ext. coefficient     4470
Abs 0.1% (=1 g/l)   0.321, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.32
This classifies the protein as unstable.



Aliphatic index: 89.60

Grand average of hydropathicity (GRAVY): -0.327