ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MANNNSDRQG LEPRVIRTLG SQALSGPSIS NRTSSSEANP HFSKNVKEAM IKTASPTPLS 

        70         80         90        100        110        120 
TPIYRIAQAC DRCRSKKTRC DGKRPQCSQC AAVGFECRIS DKLLRKAYPK GYTESLEERV 

       130        140        150        160        170        180 
RELEAENKRL LALCDIKEQQ ISLVSQSRPQ TSTDNTINGN FKHDLKDAPL NLSSTNIYLL 

       190        200        210        220        230        240 
NQTVNKQLQN GKMDGDNSGS AMSPLGAPPP PPHKDHLCDG VSCTNHLHVK PTSTSLNDPT 

       250        260        270        280        290        300 
AISFEQDEAP GLPAVKALKS MTTHQRSTQL ATLVSLSIPR STEEILFIPQ LLTRIRQIFG 

       310        320        330        340        350        360 
FNSKQCLYTV SLLSSLKNRL PAPRLLAPST STKLKEKDED KKLDDDSAFV KRFQSTNLSE 

       370        380        390        400        410        420 
FVDLKKFLIS LKFNINSFSK QSEKPANDQD DELLSLTEIK ELLHLFFKFW SNQVPILNND 

       430        440        450        460        470        480 
HFLIYFNNFV EVVKHLSTEN LETNNTTKST VTTNHEIFAL KLLMMLQMGL LVKIKMEKIK 

       490        500        510        520        530        540 
YTVPKNPKAK YARLMAYYHQ LSLIIPKNPY FLNMSTTSLP SLQLLSLASF YYLNVGDISA 

       550        560        570        580        590        600 
IYGVRGRIVS MAQQLRLHRC PSAVLSVHSN PVLQKFEQSE RRLLFWAIYY VDVFASLQLG 

       610        620        630        640        650        660 
VPRLLKDFDI ECALPISDVE YKDQLSMENE KADKKAKKIQ LQGQVSSFSL QIIRFAKILG 

       670        680        690        700        710        720 
NILDSIFKRG MMDERITSEV ALVHENALDN WRNQLPEMYY FQITVNGTVN LDEIRATNQR 

       730        740        750        760        770        780 
NTETKFDKKD IILFEKKILL LFYFLAKSMI HLPVIATKPL PKNVDNATKK KQSMFNNDSK 

       790        800        810        820        830        840 
GATNQDHMIL DVDMTSPAIR TSSSYIILQQ ATNATLTIFQ AINSMYLPLP LNVSRTLIRF 

       850        860        870        880        890        900 
SLLCARGSLE YTKGGALFLD NKNLLLDTIK DIENDRLLDL PGIASWHTLK LFDMSINLLL 

       910        920        930        940        950        960 
KAPNVKVERL DKFLEKKLNY YNRLMGLPPA TTTSLKPLFG SQSKNSLENR QRTPNVKREN 

       970        980        990       1000       1010       1020 
PEHEYLYGND SNNNNNSEAG HSPMTNTTNG NKRLKYEKDA KRNAKDGGIS KGENAHNFQN 

      1030       1040       1050       1060       1070       1080 
DTKKNMSTSN LFPFSFSNTD LTALFTHPEG PNCTNTNNGN VDVCNRASTD ATDANIENLS 

      1090       1100       1110       1120       1130       1140 
FLNMAPFLQT GNSNIGQNTI ENKPMHMDAI FSLPSNLDLM KDNMDSKPEQ LEPVIKQNPE 

      1150       1160       1170       1180       1190       1200 
NSKNNQFHQK GKSTNMEKNN LSFNNKSNYS LTKLMRLLNN DNSFSNISIN NFLYQNDQNS 

      1210       1220       1230       1240       1250       1260 
ASADPGTNKK AVTNAGANFK PPSTGSNTSQ GSILGSTKHG MDNCDFNDLG NFNNFMTNVN 

      1270       1280       1290       1300       1310       1320 
YSGVDYDYIV DASLGLAPLL VDTPDISNTN TTSTTSNRSK NSIILDTTFN DDLDRSRMNA 

      1330       1340       1350       1360       1370       1380 
REVLNPTDSI LSQGMVSSVS TRNTSNQRSL SSGNDSKGDS SSQENSKSAT GNQLDTPSTL 

      1390       1400       1410       1420       1430 
FQMRRTSSGP SASHRGPRRP QKNRYNTDRS KSSGGGSSNT DNVSDLFQWQ NAK 

References and documentation are available.

Number of amino acids: 1433

Molecular weight: 160485.47

Theoretical pI: 9.13

Amino acid composition: 
Ala (A)  75	  5.2%
Arg (R)  63	  4.4%
Asn (N) 143	 10.0%
Asp (D)  83	  5.8%
Cys (C)  16	  1.1%
Gln (Q)  63	  4.4%
Glu (E)  60	  4.2%
Gly (G)  59	  4.1%
His (H)  26	  1.8%
Ile (I)  71	  5.0%
Leu (L) 159	 11.1%
Lys (K) 107	  7.5%
Met (M)  37	  2.6%
Phe (F)  60	  4.2%
Pro (P)  70	  4.9%
Ser (S) 150	 10.5%
Thr (T)  98	  6.8%
Trp (W)   5	  0.3%
Tyr (Y)  34	  2.4%
Val (V)  54	  3.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 143
Total number of positively charged residues (Arg + Lys): 170

Atomic composition:

Carbon      C	      7014
Hydrogen    H	     11224
Nitrogen    N	      1992
Oxygen      O	      2208
Sulfur      S	        53

Formula: C7014H11224N1992O2208S53
Total number of atoms: 22491

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    79160
Abs 0.1% (=1 g/l)   0.493, assuming all pairs of Cys residues form cystines


Ext. coefficient    78160
Abs 0.1% (=1 g/l)   0.487, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.27
This classifies the protein as stable.



Aliphatic index: 78.76

Grand average of hydropathicity (GRAVY): -0.568