ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MANPTTGKSS IRAKLSNSSL SNLFKKNKNK RQREETEEQD NEDKDESKNQ DENKDTQLTP 

        70         80         90        100        110        120 
RKRRRLTKEF EEKEARYTNE LPKELRKYRP KGFRFNLPPT DRPIRIYADG VFDLFHLGHM 

       130        140        150        160        170        180 
KQLEQCKKAF PNVTLIVGVP SDKITHKLKG LTVLTDKQRC ETLTHCRWVD EVVPNAPWCV 

       190        200        210        220        230        240 
TPEFLLEHKI DYVAHDDIPY VSADSDDIYK PIKEMGKFLT TQRTNGVSTS DIITKIIRDY 

       250        260        270        280        290        300 
DKYLMRNFAR GATRQELNVS WLKKNELEFK KHINEFRSYF KKNQTNLNNA SRDLYFEVRE 

       310        320        330        340        350        360 
ILLKKTLGKK LYSKLIGNEL KKQNQRQRKQ NFLDDPFTRK LIREASPATE FANEFTGENS 

       370        380        390        400        410        420 
TAKSPDDNGN LFSQEDDEDT NSNNTNTNSD SDSNTNSTPP SEDDDDNDRL TLENLTQKKK 


QSAN 

References and documentation are available.

Number of amino acids: 424

Molecular weight: 49406.19

Theoretical pI: 8.80

Amino acid composition: 
Ala (A)  16	  3.8%
Arg (R)  28	  6.6%
Asn (N)  38	  9.0%
Asp (D)  35	  8.3%
Cys (C)   4	  0.9%
Gln (Q)  17	  4.0%
Glu (E)  34	  8.0%
Gly (G)  13	  3.1%
His (H)   7	  1.7%
Ile (I)  17	  4.0%
Leu (L)  37	  8.7%
Lys (K)  47	 11.1%
Met (M)   4	  0.9%
Phe (F)  19	  4.5%
Pro (P)  19	  4.5%
Ser (S)  27	  6.4%
Thr (T)  34	  8.0%
Trp (W)   3	  0.7%
Tyr (Y)  11	  2.6%
Val (V)  14	  3.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 69
Total number of positively charged residues (Arg + Lys): 75

Atomic composition:

Carbon      C	      2154
Hydrogen    H	      3429
Nitrogen    N	       627
Oxygen      O	       690
Sulfur      S	         8

Formula: C2154H3429N627O690S8
Total number of atoms: 6908

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    33140
Abs 0.1% (=1 g/l)   0.671, assuming all pairs of Cys residues form cystines


Ext. coefficient    32890
Abs 0.1% (=1 g/l)   0.666, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 32.29
This classifies the protein as stable.



Aliphatic index: 63.02

Grand average of hydropathicity (GRAVY): -1.151