ProtParam
User-provided sequence:
10 20 30 40 50 60
MANQKQMRTQ ILITDIPSGK FTSKWPTQLE KTLFKEQFPN LQSHLQYYTP LPFLNRIIII
70 80 90 100 110 120
FDNEDDTLQV FKFLQELLAK ENSGPMKLFV TESLLNNQHP RSRSTDDAVS LQDNNLALLE
130 140 150 160 170 180
DHRNKPLLSI NTDPGVTGVD SSSLNKGGSS LSPDKSSLES PTMLKLSTDS KPFSYQEPLP
190 200 210 220 230 240
KLSRSSSSTS NLSLNRSSQT SLPSQLENKD KSASGTKCLF ASKPLGLTID TSTRSNAASC
250 260
TENDVNATAS NPPKSPSITV NEFFH
References and
documentation are available.
Number of amino acids: 265
Molecular weight: 29255.80
Theoretical pI: 6.91
Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 27
Atomic composition:
Carbon C 1279
Hydrogen H 2049
Nitrogen N 351
Oxygen O 420
Sulfur S 6
Formula: C
1279H
2049N
351O
420S
6
Total number of atoms: 4105
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 10095
Abs 0.1% (=1 g/l) 0.345, assuming all pairs of Cys residues form cystines
Ext. coefficient 9970
Abs 0.1% (=1 g/l) 0.341, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 54.66
This classifies the protein as unstable.
Aliphatic index: 76.19
Grand average of hydropathicity (GRAVY): -0.586