ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MANQRKPILP LLLEKKPVEL VKPSKHTHCE TLSLSTLDND PFNEVMYATI YVFKANGKNL 

        70         80         90        100        110        120 
DDPVSLLRKA LSELLVHYYP LSGKLMRSES NGKLQLVYLG EGVPFEVATS TLDLSSLNYI 

       130        140        150        160        170        180 
ENLDDQVALR LVPEIEIDYE SNVCYHPLAL QVTKFACGGF TIGTALTHAV CDGYGVAQII 

       190        200        210        220        230        240 
HALTELAAGK TEPSVKSVWQ RERLVGKIDN KPGKVPGSHI DGFLATSAYL PTTDVVTETI 

       250        260        270        280        290        300 
NIRAGDIKRL KDSMMKECEY LKESFTTYEV LSSYIWKLRS RALKLNPDGI TVLGVAVGIR 

       310        320        330        340        350        360 
HVLDPPLPKG YYGNAYIDVY VELTVRELEE SSISNIANRV KKAKKTAYEK GYIEEELKNT 

       370        380        390        400        410        420 
ERLMRDDSMF EGVSDGLFFL TDWRNIGWFG SMDFGWNEPV NLRPLTQRES TVHVGMILKP 

       430        440        450        460 
SKSDPSMEGG VKVIMKLPRD AMVEFKREMA TMKKLYFGDT N 

References and documentation are available.

Number of amino acids: 461

Molecular weight: 51750.65

Theoretical pI: 6.20

Amino acid composition: 
Ala (A)  25	  5.4%
Arg (R)  20	  4.3%
Asn (N)  20	  4.3%
Asp (D)  25	  5.4%
Cys (C)   5	  1.1%
Gln (Q)   7	  1.5%
Glu (E)  35	  7.6%
Gly (G)  31	  6.7%
His (H)   9	  2.0%
Ile (I)  23	  5.0%
Leu (L)  53	 11.5%
Lys (K)  36	  7.8%
Met (M)  14	  3.0%
Phe (F)  14	  3.0%
Pro (P)  23	  5.0%
Ser (S)  31	  6.7%
Thr (T)  28	  6.1%
Trp (W)   5	  1.1%
Tyr (Y)  20	  4.3%
Val (V)  37	  8.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 60
Total number of positively charged residues (Arg + Lys): 56

Atomic composition:

Carbon      C	      2324
Hydrogen    H	      3689
Nitrogen    N	       607
Oxygen      O	       688
Sulfur      S	        19

Formula: C2324H3689N607O688S19
Total number of atoms: 7327

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    57550
Abs 0.1% (=1 g/l)   1.112, assuming all pairs of Cys residues form cystines


Ext. coefficient    57300
Abs 0.1% (=1 g/l)   1.107, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.57
This classifies the protein as stable.



Aliphatic index: 92.99

Grand average of hydropathicity (GRAVY): -0.226