ProtParam
User-provided sequence:
10 20 30 40 50 60
MANRIEQITV PDLGGASEVD VIEVLVKPGD TVAKEDGLIT LEGDKASMDV PSPLAGTIKE
70 80 90 100 110 120
LQVKVGDKVK EGDKILTLEM SAEEEKPAKE EKKESEKSEK LEEEGKKEKE EKSEPKEEKK
130 140 150 160 170 180
KATEIKEYEA EEVEGFGTSV HAGPAVRRIA REFGIDLTKI KGTGQKDRIL KEDVQKFVKE
190 200 210 220 230 240
QLKVAEGKSG IGFPPAPKID FKKFGAIEEK PLSKIKKATG VNLSRNWMTI PHVTQFGEAD
250 260 270 280 290 300
ITELQAFRQS QKEYAAKQNV RLTPLVFIIK AVVNALKEFP HFNASLDPTG EHLILKKYFH
310 320 330 340 350 360
IGVAVDTPEG LVVPVIRDAD KKGLFELAKE LGEVSEKARK KGLNMNDMQG GCFSISSLGG
370 380 390 400 410 420
IGGTAFTPII NAPEVVILGV SKMQWKPICN EAGDCKTRLM LPLSLSYDHR VIDGADGARF
430
IVYLAERLSD IRTLLL
References and
documentation are available.
Number of amino acids: 436
Molecular weight: 47973.34
Theoretical pI: 5.93
Total number of negatively charged residues (Asp + Glu): 73
Total number of positively charged residues (Arg + Lys): 68
Atomic composition:
Carbon C 2140
Hydrogen H 3490
Nitrogen N 570
Oxygen O 651
Sulfur S 11
Formula: C
2140H
3490N
570O
651S
11
Total number of atoms: 6862
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 18575
Abs 0.1% (=1 g/l) 0.387, assuming all pairs of Cys residues form cystines
Ext. coefficient 18450
Abs 0.1% (=1 g/l) 0.385, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.22
This classifies the protein as stable.
Aliphatic index: 90.34
Grand average of hydropathicity (GRAVY): -0.385