ProtParam
User-provided sequence:
10 20 30 40 50 60
MANRSEEAEY ENDSRRNHQD KKKEGVLDLV LKRPLRWLRM LIEELHWSFF FGVIIVYGVS
70 80 90 100 110 120
QGLGKGLSKV STQYYFKDEQ KIQPSQAQIY VGLIQIPWII KPLWGLLTDV VPVLGYRRRP
130 140 150 160 170 180
YFILAGFLAM ISMMVLWLHT NLHLALALSC LVAGSAGVAI ADVTIDACVT QCSISHPTLA
190 200 210 220 230 240
ADMQSLCGLC SSIGSLVGFS LSGVLVHLVG SKGVYGLLGV TAGLLVVVGM VLKESPSRSL
250 260 270 280 290 300
GRKHVNDKFL DAGSAIWKTF QYGEVWRPCL FMLLSAAVSL HIHEGMFYWY TDSKDGPSFS
310 320 330 340 350 360
KEAVGSIMSF GAIGSLVGIL LYQNFLKNFP FRNVVFWALS LSVLSGFLDL ILVLRINLKL
370 380 390 400 410 420
GLPDYFFIVV DEFVSHMISR IKWLPLLVLS SKLCPAGMEG TFFALLMSIE HIGHLVSSWG
430 440 450 460 470 480
GGVLLHALKV TRTQFDNLWL VIVIRSLLRV IPIGLVFLIP NVDSNSTILP AEMLTSRISE
490
GVDTEKIEMT ALISDEA
References and
documentation are available.
Number of amino acids: 497
Molecular weight: 55027.90
Theoretical pI: 8.09
Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 41
Atomic composition:
Carbon C 2540
Hydrogen H 4001
Nitrogen N 639
Oxygen O 677
Sulfur S 22
Formula: C
2540H
4001N
639O
677S
22
Total number of atoms: 7879
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 85745
Abs 0.1% (=1 g/l) 1.558, assuming all pairs of Cys residues form cystines
Ext. coefficient 85370
Abs 0.1% (=1 g/l) 1.551, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 37.82
This classifies the protein as stable.
Aliphatic index: 120.16
Grand average of hydropathicity (GRAVY): 0.511