ProtParam
User-provided sequence:
10 20 30 40 50 60
MANSLDRLRE HLKNGDKLVL KNNEGQSTDD ITKATMVETL SSDGSTQDSF PLNEETEIEI
70 80 90 100 110 120
DGSLVQLRII VHCWMNKDSS AADYLADCQN KQLTNVSFLQ RTDLINWLSG NTESSQYLKA
130 140 150 160 170 180
PGQKGETSDK VDIENKTLAG ELSTVKSTTS ASLENDSEVS DPVVVETMKH ERILVDHNSA
190 200 210 220 230 240
LRGAKPINFG YLIKDAELKL VQSIKGSLRG SKLPPGHKGA HGRISKTNGS SGGPRKDPII
250 260 270 280 290 300
LIPSAASSIL TVANIKQFLL ESKYVNPRNL PSVPNGLVNI EKNFERISRP IRFIIVDNTR
310 320 330 340 350 360
MFTKPEYWDR VVAIFTTGHT WQFNNYQWNS PQELFQRCKG YYFHFAGDSV PQHVQQWNVE
370 380 390
KVELDKNKRF KDVEVVRYFW HSLEKELISR GYR
References and
documentation are available.
Number of amino acids: 393
Molecular weight: 44456.16
Theoretical pI: 7.72
Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 50
Atomic composition:
Carbon C 1967
Hydrogen H 3110
Nitrogen N 554
Oxygen O 605
Sulfur S 8
Formula: C
1967H
3110N
554O
605S
8
Total number of atoms: 6244
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 53525
Abs 0.1% (=1 g/l) 1.204, assuming all pairs of Cys residues form cystines
Ext. coefficient 53400
Abs 0.1% (=1 g/l) 1.201, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 34.12
This classifies the protein as stable.
Aliphatic index: 82.80
Grand average of hydropathicity (GRAVY): -0.566