ProtParam
User-provided sequence:
10 20 30 40 50 60
MANSLRTLFS VSTHGVFLNK RSSYRVRKVF VGMPLRICSE IPRFVSVSCI RSDMCGIMML
70 80 90 100 110 120
GKDVHDLLET SSGGNVEKGF LRWRNGGGMY HRSALIDSSA LVEFGAVVHQ EAILGAEVHI
130 140 150 160 170 180
GSNTVIGSSV KIGPSTKIGN CSIGDLCVIH NGVCIGQDGF GFYVDDNGNM VKKPQTLNVK
190 200 210 220 230 240
IGNRVEIGAN TCIDRGSWRD TVIGDDTKID NLVQIGHNVI IGKCCLFCGQ VGIAGSAEIG
250 260 270 280 290
DFVALGGRVA VRDHVSIVSK VRLAANSCVT KNITEPGDYG GFPAQEETIT YSVCQTQED
References and
documentation are available.
Number of amino acids: 299
Molecular weight: 32018.65
Theoretical pI: 7.05
Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 29
Atomic composition:
Carbon C 1391
Hydrogen H 2238
Nitrogen N 402
Oxygen O 426
Sulfur S 19
Formula: C
1391H
2238N
402O
426S
19
Total number of atoms: 4476
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 19200
Abs 0.1% (=1 g/l) 0.600, assuming all pairs of Cys residues form cystines
Ext. coefficient 18450
Abs 0.1% (=1 g/l) 0.576, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 34.98
This classifies the protein as stable.
Aliphatic index: 92.78
Grand average of hydropathicity (GRAVY): 0.078